Pathogen-driven CRISPR screens identify TREX1 as a regulator of DNA self-sensing during influenza virus infection
Host: pathogen interactions dictate the outcome of infection, yet the limitations of current
approaches leave large regions of this interface unexplored. Here, we develop a novel …
approaches leave large regions of this interface unexplored. Here, we develop a novel …
[HTML][HTML] Genome-wide CRISPR/Cas9 screen identifies host factors essential for influenza virus replication
The emergence of influenza A viruses (IAVs) from zoonotic reservoirs poses a great threat to
human health. As seasonal vaccines are ineffective against zoonotic strains, and newly …
human health. As seasonal vaccines are ineffective against zoonotic strains, and newly …
Visualization and prediction of CRISPR incidence in microbial trait-space to identify drivers of antiviral immune strategy
JL Weissman, RMR Laljani, WF Fagan… - The ISME …, 2019 - academic.oup.com
Bacteria and archaea are locked in a near-constant battle with their viral pathogens. Despite
previous mechanistic characterization of numerous prokaryotic defense strategies, the …
previous mechanistic characterization of numerous prokaryotic defense strategies, the …
Persisting viral sequences shape microbial CRISPR-based immunity
Well-studied innate immune systems exist throughout bacteria and archaea, but a more
recently discovered genomic locus may offer prokaryotes surprising immunological …
recently discovered genomic locus may offer prokaryotes surprising immunological …
A genome-wide CRISPR screen in primary immune cells to dissect regulatory networks
Finding the components of cellular circuits and determining their functions systematically
remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled …
remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled …
[HTML][HTML] Exploration of bacterial bottlenecks and Streptococcus pneumoniae pathogenesis by CRISPRi-Seq
Streptococcus pneumoniae is an opportunistic human pathogen that causes invasive
diseases, including pneumonia, with greater health risks upon influenza A virus (IAV) co …
diseases, including pneumonia, with greater health risks upon influenza A virus (IAV) co …
The dynamic interplay of host and viral enzymes in type III CRISPR-mediated cyclic nucleotide signalling
Cyclic nucleotide second messengers are increasingly implicated in prokaryotic anti-viral
defence systems. Type III CRISPR systems synthesise cyclic oligoadenylate (cOA) upon …
defence systems. Type III CRISPR systems synthesise cyclic oligoadenylate (cOA) upon …
A CRISPR toolbox to study virus–host interactions
Viruses depend on their hosts to complete their replication cycles; they exploit cellular
receptors for entry and hijack cellular functions to replicate their genome, assemble progeny …
receptors for entry and hijack cellular functions to replicate their genome, assemble progeny …
Illuminating host-mycobacterial interactions with genome-wide CRISPR knockout and CRISPRi screens
Existing antibiotics are inadequate to defeat tuberculosis (TB), a leading cause of death
worldwide. We sought potential targets for host-directed therapies (HDTs) by investigating …
worldwide. We sought potential targets for host-directed therapies (HDTs) by investigating …
Genome-scale CRISPR screens identify host factors that promote human coronavirus infection
M Grodzki, AP Bluhm, M Schaefer, A Tagmount… - Genome medicine, 2022 - Springer
Background The COVID-19 pandemic has resulted in 275 million infections and 5.4 million
deaths as of December 2021. While effective vaccines are being administered globally …
deaths as of December 2021. While effective vaccines are being administered globally …