Metabolic engineering to expand the substrate spectrum of Pseudomonas putida toward sucrose

H Löwe, L Schmauder, K Hobmeier… - …, 2017 - Wiley Online Library
Sucrose is an important disaccharide used as a substrate in many industrial applications. It
is a major component of molasses, a cheap by‐product of the sugar industry. Unfortunately …

Engineering sucrose metabolism in Pseudomonas putida highlights the importance of porins

H Löwe, P Sinner, A Kremling… - Microbial …, 2020 - Wiley Online Library
Using agricultural wastes as a substrate for biotechnological processes is of great interest in
industrial biotechnology. A prerequisite for using these wastes is the ability of the industrially …

Succinic acid production from sucrose and molasses by metabolically engineered E. coli using a cell surface display system

J Ma, F Li, R Liu, L Liang, Y Ji, C Wei, M Jiang… - Biochemical …, 2014 - Elsevier
To achieve sucrose-metabolizing capability, different sucrose utilization operons have been
introduced into E. coli that cannot utilize sucrose. However, these engineered strains still …

Functional implementation of a linear glycolysis for sugar catabolism in Pseudomonas putida

A Sánchez-Pascuala, L Fernández-Cabezón… - Metabolic …, 2019 - Elsevier
The core metabolism for glucose assimilation of the soil bacterium and platform strain
Pseudomonas putida KT2440 has been reshaped from the native, cyclically-operating …

[HTML][HTML] A synthetic C2 auxotroph of Pseudomonas putida for evolutionary engineering of alternative sugar catabolic routes

NT Wirth, N Gurdo, N Krink, À Vidal-Verdú, S Donati… - Metabolic …, 2022 - Elsevier
Acetyl-coenzyme A (AcCoA) is a metabolic hub in virtually all living cells, serving as both a
key precursor of essential biomass components and a metabolic sink for catabolic pathways …

A cyclic metabolic network in Pseudomonas protegens Pf-5 prioritizes the Entner-Doudoroff pathway and exhibits substrate hierarchy during carbohydrate co …

RA Wilkes, CM Mendonca… - Applied and environmental …, 2019 - Am Soc Microbiol
The genetic characterization of Pseudomonas protegens Pf-5 was recently completed.
However, the inferred metabolic network structure has not yet been evaluated …

Sucrose utilisation in bacteria: genetic organisation and regulation

SJ Reid, VR Abratt - Applied microbiology and biotechnology, 2005 - Springer
Sucrose is the most abundant disaccharide in the environment because of its origin in
higher plant tissues, and many Eubacteria possess catalytic enzymes, such as the sucrose-6 …

Transcriptional modulation of transport-and metabolism-associated gene clusters leading to utilization of benzoate in preference to glucose in Pseudomonas putida …

A Choudhary, A Modak, SK Apte… - Applied and …, 2017 - Am Soc Microbiol
The effective elimination of xenobiotic pollutants from the environment can be achieved by
efficient degradation by microorganisms even in the presence of sugars or organic acids …

A metabolic widget adjusts the phosphoenolpyruvate-dependent fructose influx in Pseudomonas putida

M Chavarría, Á Goñi-Moreno, V de Lorenzo, PI Nikel - Msystems, 2016 - Am Soc Microbiol
Fructose uptake in the soil bacterium Pseudomonas putida occurs through a canonical
phosphoenolpyruvate (PEP)-dependent sugar transport system (PTSFru). The logic of the …

Recruiting alternative glucose utilization pathways for improving succinate production

J Tang, X Zhu, J Lu, P Liu, H Xu, Z Tan… - Applied microbiology and …, 2013 - Springer
The phosphoenolpyruvate (PEP): carbohydrate phosphotransferase system (PTS) of
Escherichia coli was usually inactivated to increase PEP supply for succinate production …