Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus …

S Tada, K Gomi, K Kitamoto, K Takahashi… - Molecular and General …, 1991 - Springer
Northern blot analysis of glucose-grown and starch-grown mycelia of Aspergillus oryzae
R11340 was conducted using the cloned Taka-amylase A (TAA) gene as a probe. The …

Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus …

Y Kanemori, K Gomi, K Kitamoto… - Bioscience …, 1999 - jstage.jst.go.jp
抄録 Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by
starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved …

Nucleotide Sequence and Expression of α-Glucosidase-encoding Gene (agdA) from Aspergillus oryzae

T Minetoki, K Gomi, K Kitamoto… - Bioscience …, 1995 - academic.oup.com
We have isolated an α-glucosidase (AGL)-encoding gene (agdA) from Aspergillus oryzae by
heterologous hybridization using the corresponding Aspergillus niger gene as a probe …

Functional elements of the promoter region of the Aspergillus oryzae glaA gene encoding glucoamylase

Y Hata, K Kitamoto, K Gomi, C Kumagai, G Tamura - Current genetics, 1992 - Springer
Analysis was made of the promoter region of the Aspergillus oryzae glaA gene encoding
glucoamylase. Northern blots using a glucoamylase cDNA as a probe indicated that the …

Deletion analysis of promoter elements of the Aspergillus oryzae agdA gene encoding α-glucosidase

T Minetoki, Y Nunokawa, K Gomi, K Kitamoto… - Current genetics, 1996 - Springer
The nucleotide sequence of a 1.5-kb fragment of the promoter region of the Aspergillus
oryzae agdA gene encoding α-glucosidase was determined. A comparison with the …

Deletion analysis of the Taka-amylase A gene promoter using a homologous transformation system in Aspergillus oryzae

K Tsuchiya, S Tada, K Gomi, K Kitamoto… - Bioscience …, 1992 - jstage.jst.go.jp
Materials and Methods Strains, plasmids, and media. A. oryzae M-2-3 (argB') was used as a
recipient strain for transformation.” Escherichia coli JM109 [rat/11,(lac-proAB), endAI …

Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae

K Gomi, T Akeno, T Minetoki, K Ozeki… - Bioscience …, 2000 - academic.oup.com
A gene, designated amyR, coding for a transcriptional activator involved in amylolytic gene
expression has been cloned from Aspergillus oryzae by screening for a clone that enabled …

Isolation of a cDNA encoding Aspergillus oryzae Taka-amylase A: evidence for multiple related genes

T Norihiro, F Makoto, N Hiroki, K Norie, T Akio… - Gene, 1989 - Elsevier
Complementary and genomic DNAs encoding Aspergillus oryzae Taka-amylase A (Taa)
were cloned and sequenced. The coding sequence of the cDNA comprised the signal …

Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae …

T Minetoki, C Kumagai, K Gomi, K Kitamoto… - Applied microbiology …, 1998 - Springer
The role of the conserved sequence region III in the promoter regions of the amylase-
encoding genes amyB, glaA and agdA of Aspergillus oryzae was examined. Introduction of …

Molecular Cloning and Characterization of the Glucoamylase Gene of Aspergillus awamori

JH Nunberg, JH Meade, G Cole… - … and Cellular Biology, 1984 - Taylor & Francis
The filamentous ascomycete Aspergillus awamori secretes large amounts of glucoamylase
upon growth in medium containing starch, glucose, or a variety of hexose sugars and sugar …