Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering

N Wada, R Ueta, Y Osakabe, K Osakabe - BMC Plant Biology, 2020 - Springer
Traditionally, generation of new plants with improved or desirable features has relied on
laborious and time-consuming breeding techniques. Genome-editing technologies have led …

Recent advances in genome editing using CRISPR/Cas9

Y Ding, H Li, LL Chen, K Xie - Frontiers in plant science, 2016 - frontiersin.org
The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …

Expanding the plant genome editing toolbox with recently developed CRISPR–Cas systems

N Wada, K Osakabe, Y Osakabe - Plant Physiology, 2022 - academic.oup.com
Since its first appearance, CRISPR–Cas9 has been developed extensively as a
programmable genome-editing tool, opening a new era in plant genome engineering …

[HTML][HTML] CRISPR/Cas9: a powerful tool for crop genome editing

G Song, M Jia, K Chen, X Kong, B Khattak, C Xie, A Li… - The crop journal, 2016 - Elsevier
The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and
represents a new generation of targeted genome editing technology that can be applied to …

Challenges facing CRISPR/Cas9-based genome editing in plants

S Son, SR Park - Frontiers in plant science, 2022 - frontiersin.org
The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …

Principles, applications, and biosafety of plant genome editing using CRISPR-Cas9

K El-Mounadi, ML Morales-Floriano… - Frontiers in plant …, 2020 - frontiersin.org
The terms genome engineering, genome editing, and gene editing, refer to modifications
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …

Modern trends in plant genome editing: an inclusive review of the CRISPR/Cas9 toolbox

A Razzaq, F Saleem, M Kanwal, G Mustafa… - International journal of …, 2019 - mdpi.com
Increasing agricultural productivity via modern breeding strategies is of prime interest to
attain global food security. An array of biotic and abiotic stressors affect productivity as well …

CRISPR/Cas9-mediated genome editing and gene replacement in plants: transitioning from lab to field

SM Schaeffer, PA Nakata - Plant Science, 2015 - Elsevier
The CRISPR/Cas9 genome engineering system has ignited and swept through the scientific
community like wildfire. Owing largely to its efficiency, specificity, and flexibility, the …

Boosting plant genome editing with a versatile CRISPR-Combo system

C Pan, G Li, AA Malzahn, Y Cheng, B Leyson… - Nature Plants, 2022 - nature.com
CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …

Progress and prospects in plant genome editing

K Yin, C Gao, JL Qiu - Nature plants, 2017 - nature.com
The emergence of sequence-specific nucleases that enable genome editing is
revolutionizing basic and applied biology. Since the introduction of CRISPR–Cas9, genome …