Image-based cell profiling is a high-throughput strategy for the quantification of phenotypic
differences among a variety of cell populations. It paves the way to studying biological …

Highlights•Image-based profiling uses hundreds of measurements of cell morphology from
images.•Samples with similar phenotypes are grouped based on this unbiased, quantitative …

With recent advances in high-throughput, automated microscopy, there has been an
increased demand for effective computational strategies to analyze large-scale, image …

Cellular phenotypes are observable characteristics of cells resulting from the interactions of
intrinsic and extrinsic chemical or biochemical factors. Image‐based phenotypic screens …

Biologists can now prepare and image thousands of samples per day using automation,
enabling chemical screens and functional genomics (for example, using RNA interference) …

Phenotypic image analysis is the task of recognizing variations in cell properties using
microscopic image data. These variations, produced through a complex web of interactions …

In morphological profiling, quantitative data are extracted from microscopy images of cells to
identify biologically relevant similarities and differences among samples based on these …

Careful visual examination of biological samples is quite powerful, but many visual analysis
tasks done in the laboratory are repetitive, tedious, and subjective. Here we describe the use …

Background Image-based screens can produce hundreds of measured features for each of
hundreds of millions of individual cells in a single experiment. Results Here, we describe …

Modern biological research increasingly relies on image data as a primary source of
information in unraveling the cellular and molecular mechanisms of life. The quantity and …