Hyperproduction of a bifunctional hybrid protein, metapyrocatechase-protein A, by gene fusion

E Kobatake, Y Ikariyama, M Aizawa, K Miwa… - Journal of …, 1990 - Elsevier
A hybrid protein between metapyrocatechase and Staphylococcal protein A was produced
by recombinant DNA techniques. A plasmid carrying the fusion gene that encodes the hybrid …

Mutagenesis of the gene encoding cytochrome c550 of Paracoccus denitrificans and analysis of the resultant physiological effects

RJ Van Spanning, C Wansell, N Harms… - Journal of …, 1990 - Am Soc Microbiol
By using synthetic oligonucleotides, the gene encoding soluble cytochrome c550 was
isolated from a genomic bank of Paracoccus denitrificans. The nucleotide sequence of the …

Organisms and substrates

I Goldberg, I Goldberg - Single Cell Protein, 1985 - Springer
Microorganisms suitable for single cell protein production are divided into four main
categories: bacteria, yeasts, molds/fungi and algae. During the last forty years, research has …

Synthesis of holo Paracoccus denitrificans cytochrome c550 requires targeting to the periplasm whereas that of holo Hydrogenobacter thermophilus cytochrome c552 …

Y Sambongi, SJ Ferguson - FEBS letters, 1994 - Elsevier
Expression from a plasmid of the complete gene, including the codons for the N-terminal
periplasmic targeting signal, for cytochrome c 550 of Paracoccus denitrificans led to the …

Pichia pastoris as an expression system for recombinant protein production

A Ciarkowska, A Jakubowska - Postepy biochemii, 2013 - europepmc.org
Pichia pastoris has become increasingly popular as a host for recombinant protein
production in recent years. P. pastoris is more cost effective and allows achieving higher …

Tryptic peptides of Penicillium vitale catalase. 1. Isolation and amino acid composition of soluble peptides

EA Kozlov, MT Kirilenko, TL Levitina… - Biopolymers and …, 1987 - biopolymers.org.ua
Biopolymers and Cell EN UK ISSN 0233-7657 (print); 1993-6842 (on-line) Home Archive
For authors Editorial Board Subscription Contacts Search Biopolym. Cell. 1987; 3(5):240-245 …

[引用][C] The expression of homologous and heterologous genes in yeast

AJ Kingsman, C Stanway, SM Kingsman - Antonie Van Leeuwenhoek, 1987 - Springer
There are two types of efficient expression vector that are used for producing and analysing
heterologous proteins in yeast. These are constitutive expression vectors that express the …

[引用][C] Using Molecular Genetics to Improve the Production of Recombinant Proteins by the Yeast Saccharomyces cerevisiae

LD Schultz, HZ Markus, KJ Hofmann… - Annals of the New …, 1994 - Wiley Online Library
The yeast Saccharomyces cerevisiae has proven to be an excellent host for the production
of a number of different recombinant proteins that have potential medical and commercial …

The amino acid sequence previously attributed to a protein kinase or a TCP1‐related molecular chaperone and co‐purified with phytochrome is a β‐glucosidase

S Gus-Mayer, H Brunner… - FEBS …, 1994 - Wiley Online Library
A 60 kDa protein (P60) co‐purified with phytochrome was identified as avenacosidase, a β‐
glucosidase which is part of the defense system of Avena sativa. An antiserum raised …

Expression of recombinant proteins in insect and mammalian cells

EA McKenzie, WM Abbott - Methods, 2018 - Elsevier
Purified recombinant proteins are key reagents in academic and industrial research. The
ability to make these proteins quickly often relies on the availability of higher eukaryotic cell …