Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry

JS Fletcher, JC Vickerman, N Winograd - Current opinion in chemical …, 2011 - Elsevier
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides a method for the
detection of native and exogenous compounds in biological samples on a cellular scale …

A new dynamic in mass spectral imaging of single biological cells

JS Fletcher, S Rabbani, A Henderson… - Analytical …, 2008 - ACS Publications
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) has unique capabilities in the
area of high-resolution mass spectrometric imaging of biological samples. The technique …

Sample preparation for 3D SIMS chemical imaging of cells

N Winograd, A Bloom - Mass Spectrometry Imaging of Small Molecules, 2015 - Springer
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is an emerging technique for the
characterization of biological systems. With the development of novel ion sources such as …

Three‐dimensional mass spectral imaging of HeLa‐M cells–sample preparation, data interpretation and visualisation

JS Fletcher, S Rabbani, A Henderson… - Rapid …, 2011 - Wiley Online Library
Time‐of‐flight secondary ion mass spectrometry (ToFSIMS) is being applied increasingly to
the study of biological systems where the chemical specificity of mass spectrometry and the …

[HTML][HTML] Latest applications of 3D ToF-SIMS bio-imaging

JS Fletcher - Biointerphases, 2015 - pubs.aip.org
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a rapidly developing
technique for the characterization of a wide range of materials. Recently, advances in …

TOF-SIMS 3D Biomolecular Imaging of Xenopus laevis Oocytes Using Buckminsterfullerene (C60) Primary Ions

JS Fletcher, NP Lockyer, S Vaidyanathan… - Analytical …, 2007 - ACS Publications
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) using buckminsterfullerene
(C60) as the primary ion source has the ability to generate chemical images of surfaces with …

A new SIMS paradigm for 2D and 3D molecular imaging of bio-systems

JS Fletcher, JC Vickerman - Analytical and bioanalytical chemistry, 2010 - Springer
With the implementation of focused primary ion beams, secondary ion mass spectrometry
(SIMS) has become a significant technique in the rapidly emerging field of mass spectral …

Uncovering new challenges in bio-analysis with ToF-SIMS

JS Fletcher, A Henderson, GX Biddulph… - Applied Surface …, 2008 - Elsevier
The introduction of cluster ion beams for routine SIMS analysis has greatly improved the
prospects for characterising biological samples. The ultimate quality of the secondary ion …

Developments in molecular SIMS depth profiling and 3D imaging of biological systems using polyatomic primary ions

JS Fletcher, NP Lockyer… - Mass spectrometry …, 2011 - Wiley Online Library
In principle mass spectral imaging has enormous potential for discovery applications in
biology. The chemical specificity of mass spectrometry combined with spatial analysis …

A concise tutorial review of TOF-SIMS based molecular and cellular imaging

P Massonnet, RMA Heeren - Journal of Analytical Atomic Spectrometry, 2019 - pubs.rsc.org
This concise tutorial review provides a description of the current state of the art in the
application of time-of flight based secondary ion mass spectrometry (TOF-SIMS) in the field …