Maturation, fertilization and complete development of porcine oocytes matured under different systems

P Coy, S Ruiz, R Romar, I Campos, J Gadea - Theriogenology, 1999 - Elsevier
This study was designed 1) to determine the effectiveness of 2 in vitro maturation systems
commonly employed to produce nuclear and cytoplasmicly mature pig oocytes, 2) to assess …

Effects of oocyte maturation media on development of pig embryos produced by in vitro fertilization

WH Wang, LR Abeydeera, TC Cantley… - Reproduction, 1997 - rep.bioscientifica.com
Few embryos derived from pig oocytes matured and inseminated in vitro are able to develop
to blastocysts in culture. The present study was conducted to examine the effects of oocyte …

Experiments to improve in vitro fertilization techniques for in vivo-matured porcine oocytes

D Rath - Theriogenology, 1992 - Elsevier
Three experiments were designed for the in vitro fertilization of in vivo matured oocytes
following different sperm treatments: In experiment I, spermatozoa (sperm rich fraction) were …

Effect of maturation media on male pronucleus formation in pig oocytes matured in vitro

M Yoshida, K Ishigaki, VG Pursel - Molecular Reproduction and …, 1992 - Wiley Online Library
The present study was carried out to examine the effect of maturation media on male
pronucleus formation of pig oocyte matured and fertilized in vitro. Follicular oocytes collected …

Asynchronous meiotic progression in porcine oocytes matured in vitro: a cause of polyspermic fertilization?

CG Grupen, H Nagashima… - Reproduction, fertility and …, 1997 - CSIRO Publishing
The progression of meiosis in porcine oocytes maturedin vitro was examined and the effect
of maturation interval on the incidence of polyspermic fertilization and embryonic …

Fertilization and blastocyst development in oocytes obtained from prepubertal and adult pigs

ES Sherrer, TJ Rathbun, DL Davis - Journal of animal science, 2004 - academic.oup.com
Polyspermic fertilization and embryo quality are important issues for the in vitro production of
pig embryos. We hypothesized that oocyte donor (prepubertal gilt vs. sow) affects …

Effect of incubation temperature on in vitro maturation of porcine oocytes: nuclear maturation, fertilisation and developmental competence

LR Abeydeera, WH Wang, RS Prather, BN Day - Zygote, 2001 - cambridge.org
The present study examined the effect of low culture temperature during in vitro maturation
(IVM) of pig oocytes on their nuclear maturation, fertilisation and subsequent embryo …

In vitro development of in vitro-matured porcine oocytes following chemical activation or in vitro fertilization

H Funahashi, TC Cantley, TT Stumpf… - Biology of …, 1994 - academic.oup.com
Porcine cumulus-oocyte complexes were cultured in BSA-free Whitten's medium or modified
Medium 199, each supplemented with porcine follicular fluid (PFF) and hormonal …

Developmental ability of porcine oocytes matured and fertilized in vitro

H Funahashi, TT Stumpf, SL Terlouw, TC Cantley… - Theriogenology, 1994 - Elsevier
The developmental abilities of porcine oocytes matured and fertilized in vitro were examined
in vivo and in vitro. Cumulus-oocyte complexes were cultured in mM199 supplemented with …

Effects of different serum supplements in maturation medium on meiotic and cytoplasmic maturation of pig oocytes

H Funahashi, BN Day - Theriogenology, 1993 - Elsevier
The temporal progression of meiotic and cytoplasmic maturation of pig oocytes cultured in a
medium supplemented with 0.4% polyvinylalcohol (PVA), 10% fetal calf serum (FCS), 10 …