Opening of apical and axillary buds of mature Douglas-fir and sugar pine trees was obtained
on a newly formulated basal medium (DCR) without growth regulators. Elongation of buds …

Numerous adventitious buds were induced when fully developed embryos of Pinus radiata
were placed on a nutrient agar medium containing cytokinin. The adventitious buds formed …

A method has been devised for the reliable production of plantlets from embryos and
seedling shoot tips of Pinus radiata D. Don (radiata pine). Buds were induced on an agar or …

Douglas fir cultures derived from excised embryos were established on a defined medium
supplemented with 5 μM each of auxins—indole-3-acetic acid (IAA) and indole-3-butyric …

Abstract Explants of Norway spruce, Scots pine, Douglas fir, Larix decidua and 16
broadleaved species or species groups grown in Czechoslovakia were cultured on 7 agar …

Shoots were regenerated from the proximal region of immature cotyledons (with the
embryonic axis removed) of Prunus persica (peach) and from the same area in mature …

Douglas fir plantlets were produced in tissue culture under defined conditions from
cotyledon explants obtained from 2-to 4-week-old seedlings. Tissue pieces were cultured on …

The shoot-forming capacity of Pinus radiata D. Don juvenile explants under tissue culture
conditions was examined. Three types of explant were used: whole embryos; cotyledons …

Vegetative propagation of woody dicots possessing one or more desirable genetic traits has
been practiced by man for centuries. Numerous species of ornamental, fruit, or nut trees …

1. INTRODUCTION AND SCOPE The factors to be considered when microculturing a plant
tissue gE, nerally can be grouped into five broad categories: 1) tissue (genotype, source …