Genome editing with engineered endonucleases is rapidly becoming a staple method in
developmental biology studies. Engineered nucleases permit random or designed genomic …

Genome editing is now a routine procedure in many mammalian genetics laboratories. The
ostensibly short but intense history of genome-editing approaches illustrates how a …

Genome editing using targetable nucleases is an emerging technology for the precise
genome modification of organisms ranging from bacteria to plants to animals. Its attraction is …

Mouse transgenesis has been instrumental in determining the function of genes in the
pathophysiology of human diseases and modification of genes by homologous …

Current technology enables the production of highly specific genome modifications with
excellent efficiency and specificity. Key to this capability are targetable DNA cleavage …

Engineered nucleases have advanced the field of gene therapy with the promise of targeted
genome modification as a treatment for human diseases. Here we discuss why engineered …

Genetic mutations in humans are associated with congenital disorders and phenotypic traits.
Gene therapy holds the promise to cure such genetic disorders, although it has suffered from …

Transgenic mice carrying site-specific genome modifications (knockout, knock-in) are of vital
importance for dissecting complex biological systems as well as for modeling human …

Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …

Recent advances in gene editing with engineered nucleases have transformed our ability to
manipulate the genome from diverse organisms for applications ranging from biomedical …