Whole-organism lineage tracing by combinatorial and cumulative genome editing
INTRODUCTION The developmental path by which a fertilized egg gives rise to the cells of a
multicellular organism is termed the cell lineage. In 1983, John Sulston and colleagues …
multicellular organism is termed the cell lineage. In 1983, John Sulston and colleagues …
Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPR–Cas9 barcodes by scGESTALT
Lineage relationships among the large number of heterogeneous cell types generated
during development are difficult to reconstruct in a high-throughput manner. We recently …
during development are difficult to reconstruct in a high-throughput manner. We recently …
Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain
The lineage relationships among the hundreds of cell types generated during development
are difficult to reconstruct. A recent method, GESTALT, used CRISPR–Cas9 barcode editing …
are difficult to reconstruct. A recent method, GESTALT, used CRISPR–Cas9 barcode editing …
[HTML][HTML] Single-cell lineage tracing by integrating CRISPR-Cas9 mutations with transcriptomic data
Recent studies combine two novel technologies, single-cell RNA-sequencing and CRISPR-
Cas9 barcode editing for elucidating developmental lineages at the whole organism level …
Cas9 barcode editing for elucidating developmental lineages at the whole organism level …
CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window
The capacity to diversify genetic codes advances our ability to understand and engineer
biological systems,. A method for continuously diversifying user-defined regions of a …
biological systems,. A method for continuously diversifying user-defined regions of a …
Simultaneous lineage tracing and cell-type identification using CRISPR–Cas9-induced genetic scars
B Spanjaard, B Hu, N Mitic, P Olivares-Chauvet… - Nature …, 2018 - nature.com
A key goal of developmental biology is to understand how a single cell is transformed into a
full-grown organism comprising many different cell types. Single-cell RNA-sequencing …
full-grown organism comprising many different cell types. Single-cell RNA-sequencing …
[HTML][HTML] Functional genetic variants revealed by massively parallel precise genome editing
A major challenge in genetics is to identify genetic variants driving natural phenotypic
variation. However, current methods of genetic mapping have limited resolution. To address …
variation. However, current methods of genetic mapping have limited resolution. To address …
CRISPR-mediated live imaging of genome editing and transcription
We report a robust, versatile approach called CRISPR live-cell fluorescent in situ
hybridization (LiveFISH) using fluorescent oligonucleotides for genome tracking in a broad …
hybridization (LiveFISH) using fluorescent oligonucleotides for genome tracking in a broad …
Karyotype engineering by chromosome fusion leads to reproductive isolation in yeast
Extant species have wildly different numbers of chromosomes, even among taxa with
relatively similar genome sizes (for example, insects),. This is likely to reflect accidents of …
relatively similar genome sizes (for example, insects),. This is likely to reflect accidents of …
[HTML][HTML] Wildtype heterogeneity contributes to clonal variability in genome edited cells
L Westermann, Y Li, B Göcmen, M Niedermoser… - Scientific reports, 2022 - nature.com
Genome editing tools such as CRISPR/Cas9 enable the rapid and precise manipulation of
genomes. CRISPR-based genome editing has greatly simplified the study of gene function …
genomes. CRISPR-based genome editing has greatly simplified the study of gene function …