Integration of Global Regulation of Two Aromatic-Responsive σ54-Dependent Systems: a Common Phenotype by Different Mechanisms

CC Sze, LMD Bernardo, V Shingler - Journal of bacteriology, 2002 - Am Soc Microbiol
Pseudomonas-derived regulators DmpR and XylR are structurally and mechanistically
related σ54-dependent activators that control transcription of genes involved in catabolism of …

Aromatic ligand binding and intramolecular signalling of the phenol‐responsive σ54‐dependent regulator DmpR

E O'Neill, LC Ng, CC Sze, V Shingler - Molecular microbiology, 1998 - Wiley Online Library
The Pseudomonas‐derived σ54‐dependent regulator DmpR has an amino‐terminal A‐
domain controlling the specificity of activation by aromatic effectors, a central C‐domain …

Identification of an effector specificity subregion within the aromatic-responsive regulators DmpR and XylR by DNA shuffling

E Skärfstad, E O'Neill, J Garmendia… - Journal of …, 2000 - Am Soc Microbiol
The Pseudomonas derived ς54-dependent regulators DmpR and XylR control the
expression of genes involved in catabolism of aromatic compounds. Binding to distinct …

σ54‐RNA polymerase controls σ70‐dependent transcription from a non‐overlapping divergent promoter

LUM Johansson, D Solera, LMD Bernardo… - Molecular …, 2008 - Wiley Online Library
Divergent transcription of a regulatory gene and a cognate promoter under its control is a
common theme in bacterial regulatory circuits. This genetic organization is found for the …

In Vivo and In Vitro Effects of Integration Host Factor at the DmpR-Regulated ς54-Dependent Po Promoter

CC Sze, AD Laurie, V Shingler - Journal of bacteriology, 2001 - Am Soc Microbiol
Transcription from the Pseudomonas CF600-derived ς54-dependent promoter Po is
controlled by the aromatic-responsive activator DmpR. Here we examine the mechanism (s) …

Novel effector control through modulation of a preexisting binding site of the aromatic-responsive ς54-dependent regulator DmpR

E O'Neill, CC Sze, V Shingler - Journal of Biological Chemistry, 1999 - ASBMB
The Pseudomonas derived ς 54-dependent DmpR activator regulates transcription of the
(methyl) phenol catabolic dmp-operon. DmpR is constitutively expressed, but its …

Roles of effectors in XylS-dependent transcription activation: intramolecular domain derepression and DNA binding

P Domínguez-Cuevas, P Marín, S Busby… - Journal of …, 2008 - Am Soc Microbiol
XylS, an AraC family protein, activates transcription from the benzoate degradation pathway
Pm promoter in the presence of a substrate effector such as 3-methylbenzoate (3MB). We …

Cooperative amino acid changes shift the response of the σ54‐dependent regulator XylR from natural m‐xylene towards xenobiotic 2,4‐dinitrotoluene

A de Las Heras, V de Lorenzo - Molecular microbiology, 2011 - Wiley Online Library
XylR is a σ54‐dependent transcriptional factor of Pseudomonas putida that activates the Pu
promoter of the TOL plasmid upon binding its natural effector, m‐xylene. The search for …

m-Xylene-Responsive Pu-PnifH Hybrid σ54 Promoters That Overcome Physiological Control in Pseudomonas putida KT2442

M Carmona, S Fernández, MJ Rodríguez… - Journal of …, 2005 - Am Soc Microbiol
ABSTRACT The sequences surrounding the− 12/− 24 motif of the m-xylene-responsive σ54
promoter Pu of the Pseudomonas putida TOL plasmid pWW0 were replaced by various DNA …

In vitro activities of an N-terminal truncated form of XylR, a σ54-dependent transcriptional activator of Pseudomonas putida

J Pérez-Martı́n, V de Lorenzo - Journal of molecular biology, 1996 - Elsevier
A truncated derivative of the XylR protein, which is able to constitutively activate the σ54-
dependentPupromoter of the TOL (toluene biodegradation) plasmid ofPseudomonas putida …