Multidimensional liquid-based separation techniques are described for maximizing the
resolution of the enormous number of peptides generated upon tryptic digestion of …

Sample complexity and dynamic range constitute enormous challenges in proteome
analysis. The back-end technology in typical proteomics platforms, namely mass …

Current solution based proteomic analysis methods are generally based on enzymatic
digestion of a protein mixture followed by separation using multidimensional liquid …

Proteomics is the study of all or part of the protein complement of genes in an organism,
often involving the analysis of complex protein/peptide samples. Such complex samples are …

The need for highly resolving separation methods that can be combined with MS has driven
the development of multidimensional LC. Although the concept is not new, Motoyama and …

This review covers recent developments towards the implementation of multi-dimensional
(MuD) liquid phase based systems for proteome investigations. Although two-dimensional …

Shotgun proteomics dominates the field of proteomics. The foundations of the strategy
consist of multiple rounds of peptide separation where chromatography provides the …

Two-dimensional liquid-chromatographic (LC) separation followed by mass spectrometric
(MS) analysis was examined for the identification of peptides in complex mixtures as an …

The system described here uses comprehensive two‐dimensional liquid chromatography to
separate the mixture of peptides resulting from the enzymatic digestion of a protein. This …

To obtain the best compromise between peak capacity and analysis time in one-
dimensional and two-dimensional (2D) liquid chromatography (LC), column technology and …