Directed culturing of microorganisms using metatranscriptomics
The vast majority of bacterial species remain uncultured, and this severely limits the
investigation of their physiology, metabolic capabilities, and role in the environment. High …
investigation of their physiology, metabolic capabilities, and role in the environment. High …
[HTML][HTML] Metatranscriptomic analysis of diverse microbial communities reveals core metabolic pathways and microbiome-specific functionality
Y Jiang, X Xiong, J Danska, J Parkinson - Microbiome, 2016 - Springer
Background Metatranscriptomics is emerging as a powerful technology for the functional
characterization of complex microbial communities (microbiomes). Use of unbiased RNA …
characterization of complex microbial communities (microbiomes). Use of unbiased RNA …
[HTML][HTML] Opportunities and challenges of using metagenomic data to bring uncultured microbes into cultivation
Although there is now an extensive understanding of the diversity of microbial life on earth
through culture-independent metagenomic DNA sequence analyses, the isolation and …
through culture-independent metagenomic DNA sequence analyses, the isolation and …
Culture-independent characterization of the digestive-tract microbiota of the medicinal leech reveals a tripartite symbiosis
PL Worthen, CJ Gode, J Graf - Applied and environmental …, 2006 - Am Soc Microbiol
Culture-based studies of the microbial community within the gut of the medicinal leech have
typically been focused on various Aeromonas species, which were believed to be the sole …
typically been focused on various Aeromonas species, which were believed to be the sole …
Use of metatranscriptomics in microbiome research
S Bashiardes… - … and biology insights, 2016 - journals.sagepub.com
The human intestinal microbiome is a microbial ecosystem that expresses as many as 100
times more genes than the human host, thereby constituting an important component of the …
times more genes than the human host, thereby constituting an important component of the …
The future of microbial metagenomics (or is ignorance bliss?)
A recent explosion in the number of studies taking advantage of the power of next-
generation sequencing to explore metagenomic or 16S rRNA taxonomic diversity of …
generation sequencing to explore metagenomic or 16S rRNA taxonomic diversity of …
Bioinformatics tools for quantitative and functional metagenome and metatranscriptome data analysis in microbes
Metagenomic and metatranscriptomic sequencing approaches are more frequently being
used to link microbiota to important diseases and ecological changes. Many analyses have …
used to link microbiota to important diseases and ecological changes. Many analyses have …
[HTML][HTML] Host matters: medicinal leech digestive-tract symbionts and their pathogenic potential
JN Marden, EA McClure, L Beka, J Graf - Frontiers in Microbiology, 2016 - frontiersin.org
Digestive-tract microbiota exert tremendous influence over host health. Host-symbiont model
systems are studied to investigate how symbioses are initiated and maintained, as well as to …
systems are studied to investigate how symbioses are initiated and maintained, as well as to …
[HTML][HTML] Generation and analysis of a mouse intestinal metatranscriptome through Illumina based RNA-sequencing
With the advent of high through-put sequencing (HTS), the emerging science of
metagenomics is transforming our understanding of the relationships of microbial …
metagenomics is transforming our understanding of the relationships of microbial …
To assemble or not to resemble—a validated comparative metatranscriptomics workflow (CoMW)
Background Metatranscriptomics has been used widely for investigation and quantification
of microbial communities' activity in response to external stimuli. By assessing the genes …
of microbial communities' activity in response to external stimuli. By assessing the genes …