Background High throughput sequencing-by-synthesis is an emerging technology that
allows the rapid production of millions of bases of data. Although the sequence reads are …

High-throughput sequencing has rapidly gained popularity for transcriptome analysis in
mammalian cells because of its ability to generate digital and quantitative information on …

Prostate cancer is a frequently diagnosed solid tumor that is originated mostly from prostate
epithelium. One of the key issues in prostate cancer research is to develop molecular …

BACKGROUND Human prostate cancer LNCaP and PC‐3 cell lines have been extensively
used to study prostate cancer progression and to develop therapeutic agents. Although …

Background RNA-seq is a powerful technique for identifying and quantifying transcription
and splicing events, both known and novel. However, given its recent development and the …

Noncoding RNAs (ncRNAs) are emerging as key molecules in human cancer, with the
potential to serve as novel markers of disease and to reveal uncharacterized aspects of …

Abstract Background Affymetrix GeneChip Array and Massively Parallel Signature
Sequencing (MPSS) are two high throughput methodologies used to profile transcriptomes …

In alternative expression analysis by sequencing (ALEXA-seq), we developed a method to
analyze massively parallel RNA sequence data to catalog transcripts and assess differential …

Sequence-based methods for transcriptome characterization have typically relied on
generation of either serial analysis of gene expression tags or expressed sequence tags …

Next-generation sequencing technologies are revolutionizing genomics research. It is now
possible to generate gigabase pairs of DNA sequence within a week without time …