The Tn 10 transpososome is a DNA processing machine in which two transposon ends, a
transposase dimer and the host protein integration host factor (IHF), are united in an …

Results. When multicopy plasmids with a set of properly oriented transposon ends are
exposed to high levels of transposase function, they give rise to new species not typical of …

Cut-and-paste transposons are important tools for mutagenesis, gene-delivery and DNA
sequencing applications. At the molecular level, the most thoroughly understood are Tn 5 …

DNA processing reactions often involve multiple components acting in concert to achieve
the desired outcome. However, it is usually difficult to know how the components …

Transposition of TnlO requires sites at the termini of the element and one essential
transposon-encoded function,“transposase”, which acts at those termini. Genetic …

The bacterial transposon Tn7 is capable of high-frequency transposition to a specific site in
the Escherichia coli chromosome, attTn7, and of low-frequency transposition to sites other …

TnlO rarely transposes, primarily because its ISlO-encoded transposase protein is
synthesized infrequently. Since the 5'end of the transposase gene is immediately adjacent to …

The bacterial transposon Tn 7 inserts at high frequency into a specific site called att Tn 7,
which is present in the chromosomes of many bacteria. We show here that transcription of a …

The transposase encoded by the tnpA gene of Tn3 is a protein specifically required for Tn3
transposition. We have purified it to homogeneity from an Escherichia coli strain containing a …