In vitro morphogenesis and differentiation of human intestinal epithelium in a gut-on-a-chip

W Shin, HJ Kim - Intestinal Differentiated Cells: Methods and Protocols, 2023 - Springer
The establishment of a three-dimensional (3D) epithelial structure and cytodifferentiation in
vitro is necessary to recapitulate in vivo-relevant structure and function of the human …

[HTML][HTML] 3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert

W Shin, HJ Kim - Nature protocols, 2022 - nature.com
Human intestinal morphogenesis establishes 3D epithelial microarchitecture and spatially
organized crypt–villus characteristics. This unique structure is necessary to maintain …

Gut-on-a-Chip microenvironment induces human intestinal cells to undergo villus differentiation

HJ Kim, DE Ingber - Integrative Biology, 2013 - academic.oup.com
Existing in vitro models of human intestinal function commonly rely on use of established
epithelial cell lines, such as Caco-2 cells, which form polarized epithelial monolayers but fail …

Author Spotlight: Development and Application of a Canine IBD Gut-on-a-Chip Model for 3D Intestinal Morphogenesis Studies

I Nagao, M Nakazawa, YM Ambrosini - JoVE (Journal of Visualized …, 2024 - jove.com
Canine intestines possess similarities in anatomy, microbiology, and physiology to those of
humans, and dogs naturally develop spontaneous intestinal disorders similar to humans …

Single-cell transcriptomics elucidates in vitro reprogramming of human intestinal epithelium cultured in a physiodynamic gut-on-a-chip

W Shin, Z Su, SS Yi, HJ Kim - bioRxiv, 2021 - biorxiv.org
The microphysiological human gut-on-a-chip has demonstrated in vivo-relevant cellular
fidelity of intestinal epithelium compared to its cultures in a static condition,. Microfluidic …

[HTML][HTML] Organ-on-chip approaches for intestinal 3D in vitro modeling

J Pimenta, R Ribeiro, R Almeida, PF Costa… - Cellular and Molecular …, 2022 - Elsevier
The intestinal epithelium has one of the highest turnover rates in the human body, which is
supported by intestinal stem cells. Culture models of intestinal physiology have been …

Advances in reconstructing intestinal functionalities in vitro: from two/three dimensional-cell culture platforms to human intestine-on-a-chip

L Wang, J Wu, J Chen, W Dou, Q Zhao, J Han, J Liu… - Talanta, 2021 - Elsevier
Standard two/three dimensional (2D/3D)-cell culture platforms have facilitated the
understanding of the communications between various cell types and their …

[HTML][HTML] Bioengineered human colon organoids with in vivo-like cellular complexity and function

O Mitrofanova, M Nikolaev, Q Xu, N Broguiere, I Cubela… - Cell Stem Cell, 2024 - cell.com
Organoids and organs-on-a-chip have emerged as powerful tools for modeling human gut
physiology and disease in vitro. Although physiologically relevant, these systems often lack …

[HTML][HTML] Contributions of the microbiome to intestinal inflammation in a gut-on-a-chip

MS Jeon, YY Choi, SJ Mo, JH Ha, YS Lee, HU Lee… - Nano …, 2022 - Springer
The intestinal microbiome affects a number of biological functions of the organism. Although
the animal model is a powerful tool to study the relationship between the host and microbe …

Combining human organoids and organ-on-a-chip technology to model intestinal region-specific functionality

G Kulkarni, A Apostolou, L Ewart, C Lucchesi… - JoVE (Journal of …, 2022 - jove.com
The intestinal mucosa is a complex physical and biochemical barrier that fulfills a myriad of
important functions. It enables the transport, absorption, and metabolism of nutrients and …