Effect of exogenous carbohydrates in a serum-free culture medium on the development of in vitro matured and fertilized porcine embryos

Y Park, J Hong, H Yong, J Lim, E Lee - Zygote, 2005 - cambridge.org
This study was conducted to examine the effect of energy substrates in a serum-free culture
medium on in vitro development of porcine embryos. Presumptive zygotes derived from in …

Improved in vitro development of porcine embryos with different energy substrates and serum

H Kim, G Lee, S Hyun, S Lee, D Nam, Y Jeong, S Kim… - Theriogenology, 2004 - Elsevier
The effect of replacing 5.5 mM glucose in North Carolina State University (NCSU)-23
medium with 0.5 mM pyruvate/5.0 mM lactate on porcine IVF embryo development was …

Effect of replacement of pyruvate/lactate in culture medium with glucose on preimplantation development of porcine embryos in vitro

NWK Karja, S Medvedev, A Onishi… - … of Reproduction and …, 2004 - jstage.jst.go.jp
The effect of glucose supplementation at different times in in vitro culture on the
developmental competence of in vitro produced (IVP) porcine embryos was examined. In …

The Effect of Energy Substrate Concentration and Amino Acids on the In Vitro Development of Preimplantation Porcine Embryos

LFS Beebe, S McIlfactrick, MB Nottle - Cloning and Stem Cells, 2007 - liebertpub.com
As the pig becomes increasingly used for biomedical research, an effective and efficient in
vitro culture system is essential. This study aimed to improve the commonly used porcine …

Embryo development and sex ratio of in vitro-produced porcine embryos are affected by the energy substrate and hyaluronic acid added to the culture medium

E Torner, E Bussalleu, MD Briz, M Yeste… - Reproduction, Fertility …, 2014 - CSIRO Publishing
In the present study, the effects of replacing glucose with pyruvate–lactate and
supplementing these in vitro culture (IVC) media with hyaluronic acid (HA) on porcine …

Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium

JH Kim, H Funahashi, K Niwa, K Okuda - Theriogenology, 1993 - Elsevier
Three experiments were conducted in which 2-cell bovine embryos were prepared from
oocytes, obtained from abattoir ovaries, by in-vitro maturation for 22 to 24 hours, followed by …

Glucose and glycine synergistically enhance the in vitro development of porcine blastocysts in a chemically defined medium

T Mito, K Yoshioka, S Yamashita… - Reproduction …, 2012 - CSIRO Publishing
In the present study, the effects of glucose and/or glycine on the in vitro development of Day
5 (Day 0= IVF) porcine blastocysts were determined. The addition of 2.5–10 mM glucose to …

Substrate utilization in porcine embryos cultured in NCSU23 and G1. 2/G2. 2 sequential culture media

AP Gandhi, M Lane, DK Gardner… - Molecular …, 2001 - Wiley Online Library
Embryo metabolism is an indicator of viability and, therefore, efficiency of the culture
medium. Currently, little is known regarding porcine embryo metabolism. The objective of …

Development and viability of in vitro derived porcine blastocysts cultured in NCSU23 and G1. 2/G2. 2 sequential medium

JE Swain, CL Bormann, RL Krisher - Theriogenology, 2001 - Elsevier
Porcine embryo development in vitro is relatively inefficient compared to other domestic
species. Currently, a single culture medium (NCSU23) is the standard for porcine in vitro …

Advanced in vitro production of pig blastocysts obtained through determining the time for glucose supplementation

S Medvedev, A Onishi, DI Fuchimoto… - … of Reproduction and …, 2004 - jstage.jst.go.jp
The objective was to determine the effect of glucose supplementation on development (to
the blastocyst stage) of in vitro matured (IVM) porcine oocytes that were either in vitro …