To the Editor—The field of genome editing is advancing rapidly1, most recently exemplified
by the advent of base editors that enable changing single nucleotides in a predictable …

The combined effect of multiple mutations on protein function is hard to predict; thus, the
ability to functionally assess a vast number of protein sequence variants would be practically …

To the Editor: We recently described the genome-wide, unbiased identification of double-
strand breaks (DSBs) enabled by sequencing (GUIDE-seq) technology, a sensitive method …

Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …

Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …

CRISPR-Cas9 and related technologies enable the efficient alteration of genomic DNA at
targeted positions and are widely used in applications ranging from individual gene …

To the Editor: Analysis of raw reads from RNA sequencing (RNA-seq) makes it possible to
reconstruct complete gene structures, including multiple splice variants, without relying on …

A global observatory for gene editing Skip to main content Thank you for visiting nature.com.
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A major challenge in genetics is to identify genetic variants driving natural phenotypic
variation. However, current methods of genetic mapping have limited resolution. To address …

INTRODUCTION The developmental path by which a fertilized egg gives rise to the cells of a
multicellular organism is termed the cell lineage. In 1983, John Sulston and colleagues …