CRISPResso2 provides accurate and rapid genome editing sequence analysis
To the Editor—The field of genome editing is advancing rapidly1, most recently exemplified
by the advent of base editors that enable changing single nucleotides in a predictable …
by the advent of base editors that enable changing single nucleotides in a predictable …
Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9
GCG Choi, P Zhou, CTL Yuen, BKC Chan, F Xu… - Nature …, 2019 - nature.com
The combined effect of multiple mutations on protein function is hard to predict; thus, the
ability to functionally assess a vast number of protein sequence variants would be practically …
ability to functionally assess a vast number of protein sequence variants would be practically …
[HTML][HTML] Open-source guideseq software for analysis of GUIDE-seq data
To the Editor: We recently described the genome-wide, unbiased identification of double-
strand breaks (DSBs) enabled by sequencing (GUIDE-seq) technology, a sensitive method …
strand breaks (DSBs) enabled by sequencing (GUIDE-seq) technology, a sensitive method …
Precision genome editing using cytosine and adenine base editors in mammalian cells
Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …
treating genetic diseases. Our laboratory developed base editing to enable precise and …
Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering
AD Garst, MC Bassalo, G Pines, SA Lynch… - Nature …, 2017 - nature.com
Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
CrispRVariants charts the mutation spectrum of genome engineering experiments
CRISPR-Cas9 and related technologies enable the efficient alteration of genomic DNA at
targeted positions and are widely used in applications ranging from individual gene …
targeted positions and are widely used in applications ranging from individual gene …
Ballgown bridges the gap between transcriptome assembly and expression analysis
To the Editor: Analysis of raw reads from RNA sequencing (RNA-seq) makes it possible to
reconstruct complete gene structures, including multiple splice variants, without relying on …
reconstruct complete gene structures, including multiple splice variants, without relying on …
A global observatory for gene editing
S Jasanoff, JB Hurlbut - Nature, 2018 - nature.com
A global observatory for gene editing Skip to main content Thank you for visiting nature.com.
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[HTML][HTML] Functional genetic variants revealed by massively parallel precise genome editing
A major challenge in genetics is to identify genetic variants driving natural phenotypic
variation. However, current methods of genetic mapping have limited resolution. To address …
variation. However, current methods of genetic mapping have limited resolution. To address …
Whole-organism lineage tracing by combinatorial and cumulative genome editing
INTRODUCTION The developmental path by which a fertilized egg gives rise to the cells of a
multicellular organism is termed the cell lineage. In 1983, John Sulston and colleagues …
multicellular organism is termed the cell lineage. In 1983, John Sulston and colleagues …