Precision genome editing using cytosine and adenine base editors in mammalian cells
Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …
treating genetic diseases. Our laboratory developed base editing to enable precise and …
Base editing: advances and therapeutic opportunities
EM Porto, AC Komor, IM Slaymaker… - Nature Reviews Drug …, 2020 - nature.com
Base editing—the introduction of single-nucleotide variants (SNVs) into DNA or RNA in
living cells—is one of the most recent advances in the field of genome editing. As around …
living cells—is one of the most recent advances in the field of genome editing. As around …
Base editing: precision chemistry on the genome and transcriptome of living cells
RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …
Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction
Base editors enable targeted single-nucleotide conversions in genomic DNA. Here we show
that expression levels are a bottleneck in base-editing efficiency. We optimize cytidine (BE4) …
that expression levels are a bottleneck in base-editing efficiency. We optimize cytidine (BE4) …
Adenine transversion editors enable precise, efficient A• T-to-C• G base editing in mammalian cells and embryos
L Chen, M Hong, C Luan, H Gao, G Ru, X Guo… - Nature …, 2024 - nature.com
Base editors have substantial promise in basic research and as therapeutic agents for the
correction of pathogenic mutations. The development of adenine transversion editors has …
correction of pathogenic mutations. The development of adenine transversion editors has …
Base editing landscape extends to perform transversion mutation
Base editors have drawn considerable academic and industrial attention in recent years
because of their ability to alter single DNA bases with precision. However, the existing …
because of their ability to alter single DNA bases with precision. However, the existing …
Precision RNA base editing with engineered and endogenous effectors
LS Pfeiffer, T Stafforst - Nature Biotechnology, 2023 - nature.com
RNA base editing refers to the rewriting of genetic information within an intact RNA molecule
and serves various functions, such as evasion of the endogenous immune system and …
and serves various functions, such as evasion of the endogenous immune system and …
Dual base editor catalyzes both cytosine and adenine base conversions in human cells
X Zhang, B Zhu, L Chen, L Xie, W Yu, Y Wang… - Nature …, 2020 - nature.com
Although base editors are useful tools for precise genome editing, current base editors can
only convert either adenines or cytosines. We developed a dual adenine and cytosine base …
only convert either adenines or cytosines. We developed a dual adenine and cytosine base …
Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery
We recently developed base editing, a genome-editing approach that enables the
programmable conversion of one base pair into another without double-stranded DNA …
programmable conversion of one base pair into another without double-stranded DNA …
Analysis and minimization of cellular RNA editing by DNA adenine base editors
Adenine base editors (ABEs) enable precise and efficient conversion of target A• T base
pairs to G• C base pairs in genomic DNA with a minimum of by-products. While ABEs have …
pairs to G• C base pairs in genomic DNA with a minimum of by-products. While ABEs have …
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