Critical evaluation of the use of hydroethidine as a measure of superoxide anion radical

L Benov, L Sztejnberg, I Fridovich - Free Radical Biology and Medicine, 1998 - Elsevier
The fluorogenic oxidation of hydroethidine (HE) to ethidium (E+) has been used as a
measure of O2−. Evaluation of this method confirms that O2−, but not O2 or H2O2, rapidly …

Release of hydrogen peroxide in response to hypoxia-reoxygenation: role of an NAD (P) H oxidase-like enzyme in endothelial cell plasma membrane.

JJ Zulueta, FS Yu, IA Hertig, VJ Thannickal… - American journal of …, 1995 - atsjournals.org
The dynamics and mechanisms of extracellular release of hydrogen peroxide (H2O2) from
bovine pulmonary artery endothelial cells (EC) subjected to anoxia, hypoxia, and hypoxia …

Use and abuse of exogenous H2O2 in studies of signal transduction

HJ Forman - Free Radical Biology and Medicine, 2007 - Elsevier
The goal of this review is to present a rationale for the use of exogenous H2O2, which has
been demonstrated to have both toxicological and physiological signaling roles. Reasons …

The fluorometric analysis of ultramicro quantities of hydrogen peroxide

AS Keston, R Brandt - Analytical biochemistry, 1965 - Elsevier
The Fluorometric Analysis of Ultramicro Q,uantities of Hydrogen Peroxide’ Page 1 ANALYTICAL
BIOCHEMISTRY 11, 1-5 (196% The Fluorometric Analysis of Ultramicro Q,uantities of Hydrogen …

Diffusion of extracellular hydrogen peroxide into intracellular compartments of human neutrophils. Studies utilizing the inactivation of myeloperoxidase by hydrogen …

Y Ohno, JI Gallin - Journal of Biological Chemistry, 1985 - ASBMB
It is well known that catalase is transformed to nitric oxide-Fe2+-catalase by hydrogen
peroxide (H2O2) plus azide. In this report, we show that myeloperoxidase is also inactivated …

Measurement of reactive oxygen species in cardiovascular studies

S Dikalov, KK Griendling, DG Harrison - Hypertension, 2007 - Am Heart Assoc
ers, made with the highest-grade chemicals, can contain trace amounts of transition metals,
and great care should be made to minimize this when performing studies of isolated tissues …

Predominant role of catalase in the disposal of hydrogen peroxide within human erythrocytes

GF Gaetani, AM Ferraris, M Rolfo, R Mangerini… - 1996 - ashpublications.org
Purified enzymes were mixed to form a cell-free system that simulated the conditions for
removal of hydrogen peroxide within human erythrocytes. Human glutathione peroxidase …

Measurements of Oxygen In Vivo: Overview and Perspectives on Methods to Measure Oxygen Within Cells and Tissues

R Springett, HM Swartz - Antioxidants & redox signaling, 2007 - liebertpub.com
The goal of this review is to summarize the major techniques that currently are being applied
to measure oxygenation of tissues in vivo. While such data would be extremely valuable …

Hydrogen peroxide and the proliferation of BHK-21 cells

RH Burdon, D Alliangana, V Gill - Free radical research, 1995 - Taylor & Francis
Intracellular levels of H2O2 in BHK-21 cells are not static but decline progressively with cell
growth. Exposure of cells to inhibitors of catalase, or glutathione peroxidase, not only …

[HTML][HTML] The role of hydrogen peroxide in redox-dependent signaling: homeostatic and pathological responses in mammalian cells

N Di Marzo, E Chisci, R Giovannoni - Cells, 2018 - mdpi.com
Hydrogen peroxide (H2O2) is an important metabolite involved in most of the redox
metabolism reactions and processes of the cells. H2O2 is recognized as one of the main …