Expanding the range of editable targets in the wheat genome using the variants of the Cas12a and Cas9 nucleases

W Wang, B Tian, Q Pan, Y Chen, F He… - Plant biotechnology …, 2021 - Wiley Online Library
The development of CRISPR‐based editors recognizing distinct protospacer‐adjacent
motifs (PAMs), or having different spacer length/structure requirements broadens the range …

Assessment of Cas12a‐mediated gene editing efficiency in plants

JM Bernabé‐Orts, I Casas‐Rodrigo… - Plant biotechnology …, 2019 - Wiley Online Library
The CRISPR/Cas12a editing system opens new possibilities for plant genome engineering.
To obtain a comparative assessment of RNA‐guided endonuclease (RGEN) types in plants …

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize

K Lee, Y Zhang, BP Kleinstiver, JA Guo… - Plant biotechnology …, 2019 - Wiley Online Library
CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …

A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)

S Gasparis, M Kała, M Przyborowski, LA Łyżnik… - Plant methods, 2018 - Springer
Background Genome editing of monocot plants can be accomplished by using the
components of the CRISPR/Cas9 (clustered regularly interspaced short palindromic …

The CRISPR/Cas9 system and its applications in crop genome editing

A Bao, DJ Burritt, H Chen, X Zhou, D Cao… - Critical reviews in …, 2019 - Taylor & Francis
The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR
associated protein9) system is an RNA-guided genome editing tool that consists of a Cas9 …

[PDF][PDF] CRISPR/CasΦ2‐mediated gene editing in wheat and rye

S Zhao, X Han, Y Zhu, Y Han, H Liu… - Journal of Integrative …, 2024 - researchgate.net
The recent advancements in developing the CRISPR/Cas9 system and various derivative
tools (eg, base editors) have accelerated basic plant science research and crop …

The power of CRISPR‐Cas9‐induced genome editing to speed up plant breeding

HX Cao, W Wang, HTT Le… - International journal of …, 2016 - Wiley Online Library
Genome editing with engineered nucleases enabling site‐directed sequence modifications
bears a great potential for advanced plant breeding and crop protection. Remarkably, the …

[HTML][HTML] CRISPR/Cas9 platforms for genome editing in plants: developments and applications

X Ma, Q Zhu, Y Chen, YG Liu - Molecular plant, 2016 - cell.com
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9
(Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II …

[HTML][HTML] CRISPR/Cas9: a powerful tool for crop genome editing

G Song, M Jia, K Chen, X Kong, B Khattak, C Xie, A Li… - The crop journal, 2016 - Elsevier
The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and
represents a new generation of targeted genome editing technology that can be applied to …

New hope for genome editing in cultivated grasses: CRISPR variants and application

A Riaz, F Kanwal, I Ahmad, S Ahmad, A Farooq… - Frontiers in …, 2022 - frontiersin.org
With the advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)
and CRISPR-associated protein (Cas) mediated genome editing, crop improvement has …