Discovery of two bifunctional/multifunctional cellulases by functional metagenomics

L Hu, S Zhang, S Chai, Y Lyu, S Wang… - Enzyme and Microbial …, 2023 - Elsevier
Cellulases are widely used in industry, and the usage in bioconversion of biofuels makes
cellulases more valuable. In this study, two tandem genes that encoded cellulases ZF994–1 …

Identification and characterization of a novel bifunctional cellulase/hemicellulase from a soil metagenomic library

S Chai, X Zhang, Z Jia, X Xu, Y Zhang, S Wang… - Applied Microbiology …, 2020 - Springer
Microbes, especially the uncultured microbes, have been considered as an important
resource for discovery of novel cellulases. In this study, a novel bifunctional cellulase …

Biochemical characterization of a novel monospecific endo-β-1, 4-glucanase belonging to GH family 5 from a rhizosphere metagenomic library

A Wierzbicka-Woś, R Henneberger… - Frontiers in …, 2019 - frontiersin.org
Cellulases have a broad range of different industrial applications, ranging from food and
beverages to pulp and paper and the biofuels area. Here a metagenomics based strategy …

Structure and function of a novel cellulase 5 from sugarcane soil metagenome

TM Alvarez, JH Paiva, DM Ruiz, JPLF Cairo, IO Pereira… - PloS one, 2013 - journals.plos.org
Cellulases play a key role in enzymatic routes for degradation of plant cell-wall
polysaccharides into simple and economically-relevant sugars. However, their low …

Isolation, identification and in silico study of native cellulase producing bacteria

F Kargar, M Mortazavi, M Maleki, MT Mahani… - Current …, 2021 - ingentaconnect.com
Aim: The purpose of this study was to screen the bacteria producing cellulase enzymes and
their bioinformatics studies. Background: Cellulose is a long-chain polymer of glucose that …

Microbial cellulases: An approach toward recent advances in research, their application, and future perspectives

R Sharma, S Nehra, D Kumar - Current Status and Future Scope of …, 2021 - Elsevier
Owing to the trouble of massive enzyme system and industrial prospective, microbial
cellulases have exhibited their probable proposal for research, in numerous industrial …

A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities

Y Zhou, X Wang, W Wei, J Xu, W Wang, Z Xie… - Biotechnology for …, 2016 - Springer
Background Cellulose, an abundant and renewable polysaccharides, constitutes the largest
resource for bioconversion of biofuels. Plant polysaccharides hydrolysis is catalyzed by …

Genetic diversity detection and gene discovery of novel glycoside hydrolase family 48 from soil environmental genomic DNA

M Hua, S Yu, Y Ma, S Chen, F Li - Annals of microbiology, 2018 - Springer
Sequence diversity within a family of functional enzymes provides a platform for novel gene
development and protein engineering to improve the properties of these enzymes for further …

Discovery of new cellulases from the metagenome by a metagenomics-guided strategy

C Yang, Y Xia, H Qu, AD Li, R Liu, Y Wang… - Biotechnology for …, 2016 - Springer
Background Energy shortage has become a global problem. Production of biofuels from
renewable biomass resources is an inevitable trend of sustainable development. Cellulose …

Genetic engineering/metabolic engineering approaches for improved cellulase production

A Verma, AM Nair, SS Yazdani - Current Status and Future Scope of …, 2021 - Elsevier
Abundantly available lignocellulosic biomass holds great potential as being feedstock for
production of renewable fuels and green chemicals. Therefore, the cellulolytic enzymes that …