Recombinant proteins are becoming increasingly important for industrial applications, where
Escherichia coli is the most widely used bacterial host for their production. However, the …

Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …

Since recombinant proteins are widely used in industry and in research, the need for their
low-cost production is increasing. Escherichia coli is one of the best known and most often …

The bacterium Escherichia coli is a major host for recombinant protein production of non-
glycosylated products. Depending on the expression strategy, the recombinant protein can …

Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving
tremendous attention for many diverse applications in the areas of industrial and medical …

The formation of inclusion bodies (IBs) constitute a frequent event during the production of
heterologous proteins in bacterial hosts. Although the mechanisms leading to their formation …

The efficient in vivo folding of many heterologous proteins is a major bottleneck of high level
production in bacterial hosts and simple optimisation protocols have not been available yet …

Recent advances in generating active proteins through refolding of bacterial inclusion body
proteins are summarized in conjunction with a short overview on inclusion body isolation …

Bacterial inclusion bodies (IBs) are refractile aggregates of protease-resistant misfolded
protein that often occur in recombinant bacteria upon gratuitous overexpression of cloned …

Culture conditions favouring the simulataneous formation of soluble protein and inclusion
bodies (IBs) were chosen for producing the cytoplasmic protein β-galactosidase or the …