Background Since publication in 1977 of plasmid pBR322, many breakthroughs in Biology
have depended on increasingly sophisticated vector platforms for analysis and engineering …

Synteny conservation analysis is a well-established methodology to investigate the potential
functional role of unknown prokaryotic genes. However, bioinformatic tools to reconstruct …

The rapidly increasing availability of microbial genome sequences has led to a growing
demand for bioinformatics software tools that support the functional analysis based on the …

Recent developments in sequencing technologies have given the opportunity to sequence
many bacterial genomes with limited cost and labor, compared to previous techniques …

High throughput sequencing is now fast and cheap enough to be considered part of the
toolbox for investigating bacteria, and there are thousands of bacterial genome sequences …

We present a Bacterial Expression Vector Archive (BEVA) for the modular assembly of
bacterial vectors compatible with both traditional and Golden Gate cloning, utilizing the Type …

Background The introduction of next generation sequencing approaches has caused a rapid
increase in the number of completely sequenced genomes. As one result of this …

The development of efficient DNA sequencing methods has led to the achievement of the
DNA sequence of entire genomes from (to date) 55 prokaryotes, 5 eukaryotic organisms and …

Microbial cells operate under governing constraints that limit their range of possible
functions. With the availability of annotated genome sequences, it has become possible to …

Large-scale bacterial population genetics studies are now routine due to cost-effective
Illumina short-read sequencing. However, analysing plasmid content remains difficult due to …