We present a new software package called Focus that interfaces cryo-transmission electron
microscopy (cryo-EM) data collection with computer image processing. Focus creates a user …

Electron cryomicroscopy can, in principle, determine the structures of most biological
molecules but is currently limited by access, specimen preparation difficulties, and cost. We …

One limitation in electron cryo-microscopy (cryo-EM) is the inability to recover high-
resolution signal from the image-recording media at the full-resolution limit of the …

Microscopy: Cryo-EM enters a new era | eLife Skip to Content eLife logo eLife home page Menu
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Imaging a material with electrons at near-atomic resolution requires a thin specimen that is
stable in the vacuum of the transmission electron microscope. For biological samples, this …

There has been enormous progress during the last few years in the determination of three-
dimensional biological structures by single particle electron cryomicroscopy (cryoEM) …

Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades
as a technique capable of studying challenging systems that otherwise defy structural …

Cryo-electron microscopy (cryo-EM) can be used to elucidate the 3D structure of
macromolecular complexes. Driven by technological breakthroughs in electron-microscope …

Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …

Cryo-electron microscopy (cryo-EM) can now be used to determine high-resolution structural
information on a diverse range of biological specimens. Recent advances have been driven …