A pilot study evaluating the utility of commercially available antibodies for flow cytometric analysis of Panthera species lymphocytes

TT Sylvester, SDC Parsons, PD van Helden… - BMC veterinary …, 2018 - Springer
Background The immune response against tuberculosis in lions is still poorly defined and
our understanding is hampered by the lack of lion specific reagents. The process for …

Molecular characterisation and expression analysis of Interferon gamma in response to natural Chlamydia infection in the koala, Phascolarctos cinereus

M Mathew, A Pavasovic, PJ Prentis, KW Beagley… - Gene, 2013 - Elsevier
Interferon gamma (IFNγ) is a key Th1 cytokine, with a principal role in the immune response
against intracellular organisms such as Chlamydia. Along with being responsible for …

Health assessment of free-ranging endangered Australian sea lion (Neophoca cinerea) pups: effect of haematophagous parasites on haematological parameters

AD Marcus, DP Higgins, R Gray - … and Physiology Part A: Molecular & …, 2015 - Elsevier
Abstract Evaluation of the health status of free-ranging populations is important for
understanding the impact of disease on individuals and on population demography and …

Quantitative measurement of equine cytokine mRNA expression by polymerase chain reaction using target-specific standard curves

CE Swiderski, TR Klei, DW Horohov - Journal of immunological methods, 1999 - Elsevier
Quantification of cytokine mRNA using reverse transcription coupled with the polymerase
chain reaction (RT-PCR) has become a corner stone of the study of cytokine regulation …

Automated single‐strand conformation polymorphism reveals low diversity of a Major Histocompatibility Complex Class II gene in the threatened New Zealand sea …

GM Lento, CS Baker, V David, N Yuhki… - Molecular Ecology …, 2003 - Wiley Online Library
Single‐strand conformation polymorphism (SSCP) analysis of the second exon of the DQB
gene in the endemic New Zealand sea lion (Phocarctos hookeri) was automated using …

Immune response biomarkers in human and veterinary research

A Llibre, D Duffy - Comparative Immunology, Microbiology and Infectious …, 2018 - Elsevier
Biomarkers are increasingly utilised in biological research and clinical practice for diagnosis
of disease, monitoring of therapeutic prognosis, or as end points in clinical studies …

Validation of a reliable set of primer pairs for measuring gene expression by real-time quantitative RT-PCR in equine leukocytes

MD Figueiredo, CE Salter, ALP Andrietti… - Veterinary immunology …, 2009 - Elsevier
Quantification of gene expression using real-time reverse transcription quantitative PCR (RT-
qPCR) is a reliable method to monitor cellular responses to pro-inflammatory stimuli. The …

Differential Expression of Immune Response Genes in Steller Sea Lions (Eumetopias jubatus): An Indicator of Ecosystem Health?

L Bowen, B Aldridge, K Beckmen, T Gelatt, L Rea… - EcoHealth, 2006 - Springer
Abstract Characterization of the polygenic and polymorphic features of the Steller sea lion
major histocompatibility complex (MHC) provides an ideal window for evaluating …

Validation of quantitative polymerase chain reaction assays for measuring cytokine expression in equine macrophages

CA Allen, SL Payne, M Harville, N Cohen… - Journal of immunological …, 2007 - Elsevier
The study of the equine immune system and inflammatory responses, by measuring cytokine
expression, can provide important insight into disease pathogenesis in the horse. A set of …

Cytokine analysis in Atlantic bottlenose dolphins

RE Cirocco, C Bigger, M Renart, G Bossart - … and Comparative Immunology, 1997 - infona.pl
Most components of the immune system demonstrate amino acid and nucleotide sequence
homology among higher vertebrates. Cytokines, regulatory cell signalling proteins, have …