The occurrence of a single reactive serine residue in several proteolytic enzymes has
indicated that the peptide sequences containing this reactiveserine are close to, or at, the …

The synthesis of the hexadecapeptide Tyr-His-His-Phe-Phe-Asp-His-Ser-Asp-Ser-His-Phe-
Asp-Phe-His-Phe was carried out by the solid-phase method. This large peptide was …

It was found that a preparation of once recrystallized chymotrypsin (1) splits
carbobenzoxyglycyl-Z-tyrosylglycineamide with great rapidity. A sam, ple of four times …

A frequently required step in peptide synthesis is the hydrolysis of an ester function used to
block the terminal carboxylgroup. The conventional methods for accomplishing this step may …

Publisher Summary Variations in the structure of TPCK (I), the reagent initially devised as a
substrate-like alkylating agent for chymotrypsin, have led to inhibitors that either inactivate …

Reactions attending the conversion of precursors of proteolytic enzymes to the active form
are distinguished by several features of unusual interest:(a) they are enzyme-catalyzed;(b) …

The initial studies on the action of crystalline swine pepsin on synthetic substrates showed
that this enzyme hydrolyzes preferentially peptide bonds involving the imino group of L …

Publisher Summary Chymotrypsins are the products of the tryptic activation of the inactive
precursors, the chymotrypsinogens produced by the pancreas of many species. The …

A comparison of the diagonalmaps' of chymotrypsin A andtosylphenylalanyl chloromethyl
ketone'-inhibited chymotrypsin A showed that His-57 is alkylated specifically by this …

Materials and Methods DIP a-chymotrypsin was prepared as described previously (2). It was
noted that boat-shaped crystals were obtained when, prior to crystallization, the protein was …