In mammalian cells, the enzymatic pathways involved in cytoplasmic mRNA decay are
incompletely defined. In this study, we have used two approaches to disrupt activities of …

In yeast, the major mRNA degradation pathway is initiated by poly (A) tail shortening that
triggers mRNA decapping. The mRNA is then degraded by 5′-to-3′ exonucleolysis. In …

The pathway of mRNA degradation has been extensively studied in the yeast,
Saccharomyces cerevisiae, and it is now clear that many mRNAs decay by a deadenylation …

Poly (A) tails are important regulators of mRNA stability and translational efficiency.
Cytoplasmic removal of poly (A) tails by 3′→ 5′ exonucleases (deadenylation) is the rate …

The poly (A) tail shortening in mRNA, called deadenylation, is the first rate-limiting step in
eukaryotic mRNA turnover, and the polyadenylate-binding protein (PABP) appears to be …

Degradation of messenger RNAs (mRNAs) plays an essential role in modulation of gene
expression and in quality control of mRNA biogenesis. Nearly all major mRNA decay …

We have used an in vitro system that reproduces in vivo aspects of mRNA turnover to
elucidate mechanisms of deadenylation. DAN, the major enzyme responsible for poly (A) tail …

Shortening and removal of the 3′ poly (A) tail of mature mRNA by poly (A)-specific 3′
exonucleases (deadenylases) is the initial and often rate-limiting step in mRNA degradation …

The human nuclear poly (A)-binding protein PABPN1 has been implicated in the decay of
nuclear noncoding RNAs (ncRNAs). In addition, PABPN1 promotes hyperadenylation by …

Deadenylation is the major step triggering mammalian mRNA decay. One consequence of
deadenylation is the formation of nontranslatable messenger RNA (mRNA) protein …