Background Epigenetic modifications have a central role in transcriptional regulation. While
several studies using next-generation sequencing have revealed genome-wide …

Epigenetic modifications undergo drastic erasure and reestablishment after fertilization. This
reprogramming is required for proper embryonic development and cell differentiation. In …

Due to its genetic amenability coupled with advances in genome editing, zebrafish is an
excellent model to examine the function of (epi) genomic elements. Here, we repurposed the …

Simple Summary The application of CRISPR/Cas9 genome editing in fish is limited to date.
An easy-to-use, economical and effective genome editing system is greatly needed. In this …

Simple Summary CRISPR/Cas9 enables efficient mutagenesis and generation of various
knockout and knockin alleles in many species including zebrafish. However, the application …

CRISPR/Cas9 system has been used widely in animals and plants to direct mutagenesis. To
date, no such method exists for fish somatic cell lines. We describe an efficient procedure for …

Gene modifications in animal models have been greatly facilitated through the application of
targeted genome editing tools. The prokaryotic CRISPR/Cas9 type II genome editing system …

In recent years, the whole life science community has witnessed the advance and
breakthrough in the genome editing technologies, including zinc-finger nucleases (ZFNs) …

The advent of genome editing has significantly altered genetic research, including research
using the zebrafish model. To better understand the selectivity of the commonly used …

Background: A single base pair mutation in the genome can result in many congenital
disorders in humans. The recent gene editing approach using CRISPR/Cas9 has rapidly …