Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase

MJ Storrs, C Poyart-Salmeron, P Trieu-Cuot… - Journal of …, 1991 - Am Soc Microbiol
Transposon Tn916 is a 16.4-kb broad-host-range conjugative transposon originally detected
in the chromosome of Enterococcus faecalis DS16. Transposition of Tn916 and related …

Specific Binding of Integrase to the Origin of Transfer (oriT) of the Conjugative Transposon Tn916

D Hinerfeld, G Churchward - Journal of Bacteriology, 2001 - Am Soc Microbiol
Purified integrase protein (Int) of the conjugative transposon Tn 916 was shown, using
nuclease protection experiments, to bind specifically to a site within the origin of conjugal …

Interaction of Related Tn916-Like Transposons: Analysis of Excision Events Promoted by Tn916 and Tn5386 Integrases

LB Rice, LL Carias, R Hutton-Thomas… - Journal of …, 2007 - Am Soc Microbiol
In recent work, we described the excision of a large genomic region from Enterococcus
faecium D344R in which the sequence from “joint” regions suggested that excision resulted …

Specific DNA cleavage mediated by the integrase of conjugative transposon Tn916

KL Taylor, G Churchward - Journal of Bacteriology, 1997 - Am Soc Microbiol
The conjugative transposon Tn916 encodes a protein called INT (Tn916) which, based on
DNA sequence comparisons, is a member of the integrase family of site-specific …

The Frequency of Conjugative Transposition of Tn916 Is Not Determined by the Frequency of Excision

D Marra, B Pethel, GG Churchward… - Journal of …, 1999 - Am Soc Microbiol
Excision and formation of a covalently closed circular transposon molecule are required for
conjugative transposition of Tn 916 but are not the only factors that limit the frequency of …

DNA binding by the Xis protein of the conjugative transposon Tn916

CK Rudy, JR Scott, G Churchward - Journal of bacteriology, 1997 - Am Soc Microbiol
We purified the Xis protein of the conjugative transposon Tn916 and showed by nuclease
protection experiments that Xis bound specifically to sites close to each end of Tn916. These …

Conjugative transposition.

JR Scott, GG Chruchward - Annual review of microbiology, 1995 - go.gale.com
Conjugative transposons are transposable elements that can cause gram-positive bacteria
to be conjugational donors to promote antibiotic resistance. The can conjugate by moving …

Tn916 target DNA sequences bind the C-terminal domain of integrase protein with different affinities that correlate with transposon insertion frequency

F Lu, G Churchward - Journal of bacteriology, 1995 - Am Soc Microbiol
The conjugative transposon Tn916 inserts with widely different frequencies into a variety of
target sites with related nucleotide sequences. The binding of chimeric proteins, consisting …

Sequence analysis of termini of conjugative transposon Tn916

DB Clewell, SE Flannagan, Y Ike, JM Jones… - Journal of …, 1988 - Am Soc Microbiol
Transposon Tn916 is a 16.4-kilobase, broad-host-range, conjugative transposon originally
identified on the chromosome of Enterococcus (Streptococcus) faecalis DS16. Its termini …

Transposition genes of the Bacteroides mobilizable transposon Tn4555: role of a novel targeting gene

GD Tribble, AC Parker, CJ Smith - Molecular microbiology, 1999 - Wiley Online Library
Conjugative transposons have been identified in several bacterial species, most notably the
Gram‐positive Enterococci and the Gram‐negative Bacteroides. In Bacteroides species …