Effect of short periods of sperm–oocyte coincubation during in vitro fertilization on embryo development in pigs

MA Gil, M Ruiz, JM Vazquez, J Roca, BN Day… - Theriogenology, 2004 - Elsevier
The present study was conducted to determine if the efficiency of in vitro pig embryo
production could be improved by a reduction in the period of time that oocytes are exposed …

Effects of in vitro fertilization conditions on preimplantation development and quality of pig embryos

DB Koo, YJ Kim, I Yu, HN Kim, KK Lee… - Animal reproduction …, 2005 - Elsevier
The present study was to investigate the effects of in vitro fertilization conditions on in vitro
development and structural integrity of pig embryos. Porcine oocytes matured in vitro were …

Influence of sperm: oocyte ratio during in vitro fertilization of in vitro matured cumulus-intact pig oocytes on fertilization parameters and embryo development

MA Gil, M Ruiz, C Cuello, JM Vazquez, J Roca… - Theriogenology, 2004 - Elsevier
The present study was conducted to evaluate the influence of sperm: oocyte ratio during in
vitro fertilization (IVF) of in vitro matured cumulus-intact oocytes on fertilization parameters …

Effect of the volume of medium and number of oocytes during in vitro fertilization on embryo development in pigs

MA Gil, LR Abeydeera, BN Day, JM Vazquez, J Roca… - Theriogenology, 2003 - Elsevier
The present study was designed to determine the effect of the volume of medium (VM) and
the number of oocytes (NOOC) during in vitro fertilization (IVF) on embryo development in …

Effects of ultrashort gamete co-incubation time on porcine in vitro fertilization

C Alminana, MA Gil, C Cuello, I Parrilla, J Roca… - Animal reproduction …, 2008 - Elsevier
A reduction in co-incubation time has been suggested as an alternative method to reduce
polyspermic fertilization. The aim of this study was to evaluate the effect of short periods of …

Brief coincubation of gametes in porcine in vitro fertilization: role of sperm: oocyte ratio and post-coincubation medium

MA Gil, C Almiñana, C Cuello, I Parrilla, J Roca… - Theriogenology, 2007 - Elsevier
In this study, a short coincubation time of 10min was used to determine the effect of different
sperm: oocyte ratios during in vitro fertilization (IVF), and different periods of post …

Fertilization and blastocyst development in oocytes obtained from prepubertal and adult pigs

ES Sherrer, TJ Rathbun, DL Davis - Journal of animal science, 2004 - academic.oup.com
Polyspermic fertilization and embryo quality are important issues for the in vitro production of
pig embryos. We hypothesized that oocyte donor (prepubertal gilt vs. sow) affects …

Sperm concentration influences fertilization and male pronuclear formation in vitro in pigs

P Coy, E Martinez, S Ruiz, JM Vazquez, J Roca… - Theriogenology, 1993 - Elsevier
To study the effect of sperm concentration on the results of pig in vitro fertilization (IVF), 313
oocytes recovered from oviducts of prepubertal gilts after induction of ovulation were used …

Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa

LR Abeydeera, BN Day - Biology of reproduction, 1997 - academic.oup.com
The present study examined the penetrability of pig oocytes by frozen-thawed ejaculated
boar spermatozoa, prepared by the pellet method, coincubated in a modified Tris-buffered …

Differences in pronucleus formation and first cleavage following in vitro fertilization between pig oocytes matured in vivo and in vitro

J Laurincik, D Rath, H Niemann - Reproduction, 1994 - rep.bioscientifica.com
To elucidate the developmental differences occurring after in vitro fertilization (IVF) of pig
oocytes matured either in vitro (n= 1934) or in vivo (n= 1128), the present experiment …