Genome editing has emerged as a technology with a potential to revolutionize plant
breeding. In this study, we report on generating, in less than ten months, Tomelo, a non …

Pathogenic microbes are responsible for severe production losses in crops worldwide. The
use of disease resistant crop varieties can be a sustainable approach to meet the food …

Several expression systems for multiple guide RNA (gRNAs) have been developed in the
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR …

Background The use of homologous recombination to precisely modify plant genomes has
been challenging, due to the lack of efficient methods for delivering DNA repair templates to …

Key message CRISPR/Cas9 system can precisely edit genomic sequence and effectively
create knockout mutations in T0 generation watermelon plants. Abstract Genome editing …

The combined availability of whole genome sequences and genome editing tools is set to
revolutionize the field of fruit biotechnology by enabling the introduction of targeted genetic …

Genome editing by sequence-specific nucleases (SSNs) has revolutionized biology by
enabling targeted modifications of genomes. Although routine plant genome editing …

Highlights•Presented the typical workflow of a CRISPR screen in plants.•Collected and
described appropriate genome editors for large-scale editing in plant.•Envisioned a virus …

Crop improvement by inbreeding often results in fitness penalties and loss of genetic
diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by …

Key message We have developed multiplex genome editing toolkits for citrus that
significantly improve citrus genome editing efficacy. Abstract CRISPR/Cas systems have …