Aspergillus niger is a vital industrial workhouse widely used for the production of organic
acids and industrial enzymes. This fungus is a crucial cell factory due to its innate tolerance …

Aspergillus niger is an important industrial strain which has been widely used for production
of enzymes and organic acids. Genome modification of A. niger is required to further …

Aspergillus niger is an important industrial producer of enzymes due to its high capacity for
producing exocellular secretory proteins. The CRISPR/Cas9 system has been developed as …

Aspergillus, a genus of filamentous fungi, is extensively distributed in nature and plays
crucial roles in the decomposition of organic materials as an important environmental …

Objectives To develop a one-step, marker-free CRISPR/Cas9 system for highly efficient
genome editing in industrial Aspergillus niger, with a short genetic operation cycle. Results …

CRISPR technology has revolutionized fungal genetic engineering by accelerating the pace
and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas …

Background CRISPR-Cas genome editing technologies have revolutionized
biotechnological research particularly in functional genomics and synthetic biology. As an …

Aspergillus niger, as an important industrial strain, is widely used in the production of a
variety of organic acids and industrial enzymes. To excavate the greater potential of A. niger …

Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

CRISPR-Cas9 technology has been utilized in different organisms for targeted mutagenesis,
offering a fast, precise and cheap approach to speed up molecular breeding and study of …