CRISPR/Cas-based genome editing in Aspergillus niger
X Zheng, P Zheng, J Sun - Sheng wu Gong Cheng xue bao …, 2021 - europepmc.org
Aspergillus niger is a vital industrial workhouse widely used for the production of organic
acids and industrial enzymes. This fungus is a crucial cell factory due to its innate tolerance …
acids and industrial enzymes. This fungus is a crucial cell factory due to its innate tolerance …
An efficient marker-free genome editing method for Aspergillus niger
Y Shen, Z Chen, J Chen, B Zhao, J Lü… - Sheng wu Gong …, 2022 - europepmc.org
Aspergillus niger is an important industrial strain which has been widely used for production
of enzymes and organic acids. Genome modification of A. niger is required to further …
of enzymes and organic acids. Genome modification of A. niger is required to further …
Efficient genome editing in Aspergillus niger with an improved recyclable CRISPR-HDR toolbox and its application in introducing multiple copies of heterologous …
H Dong, J Zheng, D Yu, B Wang, L Pan - Journal of microbiological …, 2019 - Elsevier
Aspergillus niger is an important industrial producer of enzymes due to its high capacity for
producing exocellular secretory proteins. The CRISPR/Cas9 system has been developed as …
producing exocellular secretory proteins. The CRISPR/Cas9 system has been developed as …
[HTML][HTML] CRISPR/Cas9-Based Genome Editing and Its Application in Aspergillus Species
FJ Jin, BT Wang, ZD Wang, L Jin, P Han - Journal of Fungi, 2022 - mdpi.com
Aspergillus, a genus of filamentous fungi, is extensively distributed in nature and plays
crucial roles in the decomposition of organic materials as an important environmental …
crucial roles in the decomposition of organic materials as an important environmental …
Establishing a one-step marker-free CRISPR/Cas9 system for industrial Aspergillus niger using counter-selectable marker Ang-ace2
J Liu, J Zhu, Q Zhang, R Lv, H Liu - Biotechnology Letters, 2023 - Springer
Objectives To develop a one-step, marker-free CRISPR/Cas9 system for highly efficient
genome editing in industrial Aspergillus niger, with a short genetic operation cycle. Results …
genome editing in industrial Aspergillus niger, with a short genetic operation cycle. Results …
CRISPR-Cas9/Cas12a Systems for efficient genome editing and large genomic fragment deletions in Aspergillus niger
CRISPR technology has revolutionized fungal genetic engineering by accelerating the pace
and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas …
and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas …
[HTML][HTML] Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277
D Abdulrachman, L Eurwilaichitr, V Champreda… - BMC …, 2021 - Springer
Background CRISPR-Cas genome editing technologies have revolutionized
biotechnological research particularly in functional genomics and synthetic biology. As an …
biotechnological research particularly in functional genomics and synthetic biology. As an …
[HTML][HTML] Efficient gene deletion and replacement in Aspergillus niger by modified in vivo CRISPR/Cas9 systems
Y Zhang, L Ouyang, Y Nan, J Chu - Bioresources and Bioprocessing, 2019 - Springer
Aspergillus niger, as an important industrial strain, is widely used in the production of a
variety of organic acids and industrial enzymes. To excavate the greater potential of A. niger …
variety of organic acids and industrial enzymes. To excavate the greater potential of A. niger …
[HTML][HTML] CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective
R Song, Q Zhai, L Sun, E Huang, Y Zhang… - Applied microbiology …, 2019 - Springer
Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …
production. With the increasing number of full genomes available for fungal species, the …
Editing Aspergillus terreus using the CRISPR-Cas9 system
SY Shih, UH Mortensen, FR Chang, HY Tsai - Synthetic Biology, 2022 - academic.oup.com
CRISPR-Cas9 technology has been utilized in different organisms for targeted mutagenesis,
offering a fast, precise and cheap approach to speed up molecular breeding and study of …
offering a fast, precise and cheap approach to speed up molecular breeding and study of …