Cultured osteoblasts from chick embryo calvaria were used as a model system to investigate
the post‐translational extracellular mechanisms controlling the macroassembly of collagen …

Biomineralization involves proteins and/or other macromolecules directly in controlling the
mineral crystal nucleation/induction, growth, and maturation. To identify these proteins in …

To examine the autocrine effects that an organizing extracellular matrix has on osteoblast
precursors, we created MC3T3-E1 cell lines that stably expressed pro-α1 (I) collagen chains …

The recent development of new specific immunoassays has provided an opportunity to study
the assembly and resorption of type II and IX collagens of the extracellular matrix in …

In bone, hydroxyapatite (HA) crystals are deposited onto the type I collagen scaffold by a
mechanism that has yet to be elucidated. Bone sialoprotein (BSP) is an acidic …

We report a novel method to culture chick embryo osteoblasts in vitro. Primary cells were
grown from explants of calvaria and then cultured within alginate polymer beads. Enriched …

Chondroitin sulfate is known to inhibit formation of hydroxyapatite in solution. It is not known
whether chondroitin sulfate will prevent mineralization in a cellularly controlled environment …

Although> 80% of the mineral in mammalian bone is present in the collagen fibrils, limited
information is available about factors that determine a proper deposition of mineral. This …

Osteogenic cells express some matrix proteins at early culture intervals. The aim of this
study was to determine if, and in what proportion, cells used for plating contain bone …

To better understand the biochemistry of matrix‐forming cells, we developed a simple and
reproducible procedure for the isolation and identification by N‐terminal sequencing of …