A new assay has been developed for vesicle-vesicle fusion based upon the mixing of
intravesicular contents of two sets of vesicles. Purified firefly luciferase and MgCl 2 were …

Jan Wilschut,* Nejat Düzgüne § , § Robert Fraley, 1 and Demetrios Papahadjopoulos*
abstract: We describe an assay for following the mixing of aqueous contentsduring fusion of …

Secretory vesicles isolated from rat liver were found to fuse after exposure to Ca 2+. Vescle
fusion is characterized by the occurrence of twinned vesicles with a continuous cleavage …

The kinetics of Ca 2+-induced fusion of small unilamellar phosphatidylserine (PS) vesicles
was investigated by continuous monitoring of the mixing of vesicle lipids and, in parallel, the …

Secretory vesicles isolated from adrenal medulla were found to fuse in vitro in response to
incubation with Ca 2+. Intervesicular fusion was detected by electron microscopy and was …

Ricin, a plant toxin that binds to galactose‐terminated glycoproteins and glycolipids on the
cell surface, is internalized into endosomes before reaching the cytosol where it exerts its …

The relative kinetics of intermixing and release of liposome aqueous contents during Ca 2+-
induced membrane fusion has been investigated. Fusion was monitored by the Tb …

The effect of divalent metals on the interaction and mixing of membrane components in
vesicles prepared from acidic phospholipids has been examined using freeze-fracture …

An assay based on the fluorescent compound 2', 7'-([bis (carboxymethyl) amino] methyl)
fluorescein (calcein) has been developed to investigate vesicle fusion and lysis. The assay …

The kinetics of Ca 2+-induced fusion of both large and small phosphatidylserine vesicles
has been examined theoretically and experimentally. With the Tb/DPA assay for mixing of …