[HTML][HTML] xCas9 expands the scope of genome editing with reduced efficiency in rice
J Wang, X Meng, X Hu, T Sun, J Li… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
CRISPR-Cas9 systems have been widely used in animals and plants for genome editing,
epigenetic modification, etc.(Cong et al., 2013; Hilton et al., 2015). However, the accurate …
epigenetic modification, etc.(Cong et al., 2013; Hilton et al., 2015). However, the accurate …
[HTML][HTML] Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis
AA Malzahn, X Tang, K Lee, Q Ren, S Sretenovic… - BMC biology, 2019 - Springer
Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …
[HTML][HTML] Cas9-NG greatly expands the targeting scope of the genome-editing toolkit by recognizing NG and other atypical PAMs in rice
B Ren, L Liu, S Li, Y Kuang, J Wang, D Zhang, X Zhou… - Molecular plant, 2019 - cell.com
CRISPR technologies enabling precise genome manipulation are valuable for gene function
studies and molecular crop breeding. However, the requirement of a protospacer adjacent …
studies and molecular crop breeding. However, the requirement of a protospacer adjacent …
[HTML][HTML] Improved base editor for efficiently inducing genetic variations in rice with CRISPR/Cas9-guided hyperactive hAID mutant
B Ren, F Yan, Y Kuang, N Li, D Zhang, X Zhou, H Lin… - Molecular plant, 2018 - cell.com
Single-nucleotide polymorphism (SNP) is an abundant form of genetic variation among
individuals in plant species, which has been believed to have effects on the gene function …
individuals in plant species, which has been believed to have effects on the gene function …
CRISPR-Cas9 based plant genome editing: Significance, opportunities and recent advances
Precise genome editing is a quantum leap in the field of plant sciences. Clustered regularly
interspaced short palindromic repeats (CRISPR) and its associated Cas9 protein have …
interspaced short palindromic repeats (CRISPR) and its associated Cas9 protein have …
[HTML][HTML] CRISPR-Cas9 editing in maize: systematic evaluation of off-target activity and its relevance in crop improvement
J Young, G Zastrow-Hayes, S Deschamps… - Scientific reports, 2019 - nature.com
CRISPR-Cas9 enabled genome engineering has great potential for improving agriculture
productivity, but the possibility of unintended off-target edits has evoked some concerns …
productivity, but the possibility of unintended off-target edits has evoked some concerns …
[HTML][HTML] Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice
W Xu, W Song, Y Yang, Y Wu, X Lv, S Yuan, Y Liu… - BMC plant biology, 2019 - Springer
Background Application of the CRISPR/Cas9 system or its derived base editors enables
targeted genome modification, thereby providing a programmable tool to exploit gene …
targeted genome modification, thereby providing a programmable tool to exploit gene …
CRISPR/Cas9 activity in the rice OsBEIIb gene does not induce off-target effects in the closely related paralog OsBEIIa
Genome editing with the CRISPR/Cas9 system allows mutations to be induced at any 20-bp
target site in the genome preceded by the short protospacer adjacent motif (PAM) 5′-NGG …
target site in the genome preceded by the short protospacer adjacent motif (PAM) 5′-NGG …
[HTML][HTML] Applications of the CRISPR/Cas9 system for rice grain quality improvement: perspectives and opportunities
Grain quality improvement is a key target for rice breeders, along with yield. It is a multigenic
trait that is simultaneously influenced by many factors. Over the past few decades, breeding …
trait that is simultaneously influenced by many factors. Over the past few decades, breeding …
[HTML][HTML] A new rice breeding method: CRISPR/Cas9 system editing of the Xa13 promoter to cultivate transgene‐free bacterial blight‐resistant rice
C Li, W Li, Z Zhou, H Chen, C Xie… - Plant Biotechnology …, 2020 - ncbi.nlm.nih.gov
Dear Editor, Since the first successful completion of genome-based editing in the model
plant Arabidopsis using CRISPR/Cas9 in 2013 (Jiang et al., 2013), a large number of …
plant Arabidopsis using CRISPR/Cas9 in 2013 (Jiang et al., 2013), a large number of …