The recent development of sequence-specific nuclease systems, ie, TALENs and
CRISPR/Cas9, has made genomic targeting easier in many organisms including plants (Li …

Gene-targeting technologies using sequence specific nucleases have been widely adopted
to induce genetic modifications in both plant molecular biology research and crop …

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …

CRISPR-Cas9,-Cas12a,-Cas12b, and-Cas13 have been harnessed for genome
engineering in human and plant cells (Liu et al., 2022). However, the large size of these Cas …

Abstract Key message Using CRISPR/Cas9, we successfully deleted large fragments of the
yield-related gene DENSE AND ERECT PANICLE1 in Indica rice at relatively high frequency …

Efficient CRISPR/Cas9‐based plant genomic fragment deletions by microhomology‐mediated
end joining - PMC Back to Top Skip to main content NIH NLM Logo Access keys NCBI …

Generating mutants bearing multiple gene modifications is essential for 1 determining the
functions of gene family members with redundant functions, or for 2 analyzing epistatic …

Common wheat supplies vast amounts of dietary carbohydrate and protein for over 60% of
world population. With continued economic development, demand for high-yielding varieties …

Abstract Background The CRISPR/Cas9 system is being used for genome editing purposes
by many research groups in multiple plant species. Traditional sequencing methods to …

Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing -
PMC Back to Top Skip to main content NIH NLM Logo Access keys NCBI Homepage MyNCBI …