Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing -
PMC Back to Top Skip to main content NIH NLM Logo Access keys NCBI Homepage MyNCBI …

Various abiotic stresses, such as drought, salinity, heat, flooding, ion toxicity and radiation
are the major constraints to agricultural production. The understanding of molecular basis of …

Despite recent progress, the application of CRISPR/Cas9 in perennial plants still has many
obstacles to overcome. Our previous results with CRISPR/Cas9 in apple and pear indicated …

CRISPR–Cas9-mediated genome editing has been widely adopted for basic and applied
biological research in eukaryotic systems. While many studies consider DNA sequences of …

Since the completion of the rice (Oryza sativa) genomesequencing project, a major goal of
rice research has been the functional characterization of all annotated genetic loci in various …

Background Plant genome engineering mediated by various CRISPR-based tools requires
specific protospacer adjacent motifs (PAMs), such as the well-performed NGG, NG, and …

Main conclusion An improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-
driven Cas9 exhibits consistently high mutation efficiency in Arabidopsis and M. truncatula …

Rice (Oryza sativa L.) is the major food source for more than three billion people of the
world. In the last few decades, the classical, mutational, and molecular breeding approaches …

The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …

PAM‐relaxed Cas9 nucleases, cytosine base editors and adenine base editors are
promising tools for precise genome editing in plants. However, their genome‐wide off‐target …