Key message A method based on DNA single-strand conformation polymorphism is
demonstrated for effective genotyping of CRISPR/Cas9-induced mutants in rice. Abstract …

Genome editing has become a major tool for both functional studies and plant breeding in
several species. Besides generating knockouts through the classical CRISPR-Cas9 system …

Since its first appearance, CRISPR–Cas9 has been developed extensively as a
programmable genome-editing tool, opening a new era in plant genome engineering …

CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …

CRISPR/Cas9 genome editing relies on sgRNA-target DNA base pairing and a short
downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif …

Herbicide resistance is one of the most important traits for plant biotechnology, which is
widely used to improve agricultural efficiency by controlling weeds and also used as a …

The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and
represents a new generation of targeted genome editing technology that can be applied to …

Transient expression of CRISPR/Cas9 is an effective approach for limiting its activities and
improving its precision in genome editing. Here, we describe the heat‐shock‐inducible …

The newly developed CRISPR/Cas9-mediated base editing technology with cytosine
deaminase is capable of precisely and efficiently introducing point mutations at the target …

Mutations generated by CRISPR/Cas9 in Arabidopsis (Arabidopsis thaliana) are often
somatic and are rarely heritable. Isolation of mutations in Cas9-free Arabidopsis plants can …