Monitoring the formation of soluble immune complexes composed of idiotype and anti-idiotype antibodies by electron microscopy

KH Roux, NS Greenspan - Molecular immunology, 1994 - Elsevier
We have previously used immunoelectron microscopy (IEM) to generate a three-
dimensional map of idiotypic (Id) and isotypic epitopes on the Fab arms of HGAC 39 (Roux …

Electron microscopic study of ring-shaped, bivalent hapten, bivalent antidansyl monoclonal antibody complexes with identical variable domains but IgG1, IgG2a and …

ML Phillips, VT Oi, VN Schumaker - Molecular immunology, 1990 - Elsevier
We have studied by electron microscopy a fascinating series of antidansyl monoclonal
antibodies developed by Dangl et al.(Cytometry2, 395–401, 1982) which have the same …

Construction of an extended three-dimensional idiotope map by electron microscopic analysis of idiotope-anti-idiotope complexes.

KH Roux, WJ Monafo, JM Davie… - Proceedings of the …, 1987 - National Acad Sciences
A three-dimensional map of the positions of four idiotypic determinants (idiotopes or Ids) and
an isotypic determinant was derived by transmission electron microscopy of negatively …

Probing the idiotype/anti-idiotype antibody interaction with a set of synthetic peptide homologues

LE Brown, T McInerney, EM Anders, DO White… - Immunology letters, 1991 - Elsevier
Abstract Anti-idiotypic (anti-Id) antibodies were raised against two murine monoclonal
antibodies (mAb 1 1 and mAb 2 1) which recognise two distinct and well-characterised …

Restricted heterogeneity of antibodies to idiotypes of isologous immunoglobulins

N Sakato, SH Hall, HN Eisen - Immunochemistry, 1977 - Elsevier
To determine whether isologous anti-idiotypic antibody responses are degenerate, the
antibodies made by BALB/c mice to the idiotype of M167, an IgA, k myeloma protein of …

Exploring the Mimicry of Polysaccharide Antigens by Anti-idiotypic Antibodies: The Crystallization, Molecular Replacement, and Refinement to 2· 8 Å Resolution of an …

SV Evans, DR Rose, R To, NM Young… - Journal of molecular …, 1994 - Elsevier
The monoclonal antibody YsT9. 1 is specific for the lipopolysaccharide A antigen of Brucella
abortus. A complex formed between the Fab of YsT9. 1 (Ab1) and the Fab of its anti-idiotopic …

Importance of dynamic properties of idiotopes in interactions with anti-Id antibodies

EW Voss, KM Weidner, LK Denzin - Immunological investigations, 1992 - Taylor & Francis
The concept of fluctuating confomational substates in equilibrium characterizing variable-
regions of immunoglobulin idiotypes is discussed in terms of the dynamic properties of …

Specific antigedantibody complexes induce the in vivo production of a parallel set of nonantigen‐binding idiotype‐positive antibodies

MJ Caulfield, J Cerny - European journal of immunology, 1988 - Wiley Online Library
Immune complexes prepared with the polysaccharide antigen (PnC) extracted from
Streptococcus pneumoniae R36a and two different PnC‐specific antibodies were found to …

Immunoelectron microscopic localization of idiotypes and allotypes on immunoglobulin molecules.

KH Roux, DW Metzger - Journal of immunology (Baltimore, Md …, 1982 - journals.aai.org
We have developed a novel approach to the analysis of antigenic (allotypic and idiotypic)
determinants on intact immunoglobulin molecules. Immune complexes composed of IgG in …

Comparison of the conformations of two intact monoclonal antibodies with hinges

LJ Harris, SB Larson, E Skaletsky… - Immunological …, 1998 - Wiley Online Library
Structures of two intact monoclonal antibodies were solved by X‐ray diffraction analysis
revealing, in both cases, the dispositions of all segments, as well as the structures of the …