The complex of importin‐alpha and‐beta is essential for nuclear protein import. It binds the
import substrate in the cytosol, and the resulting trimeric complex moves through the nuclear …

Nuclear import of classical nuclear localization sequence-bearing proteins is mediated by
karyopherin α/β1 heterodimers. A second nuclear import pathway, mediated by karyopherin …

Importin α plays a pivotal role in the classical nuclear protein import pathway. Importin α
shuttles between nucleus and cytoplasm, binds nuclear localization signal-bearing proteins …

The spatial separation of DNA replication and gene transcription in the nucleus and protein
translation in the cytoplasm is a uniform principle of eukaryotic cells. This …

To enter the nucleus a protein must be chaperoned by a transport factor through the nuclear
pore complex or it must be small enough to pass through by diffusion. Although these …

We have identified a novel human karyopherin (Kap) β family member that is related to
human Crm1 and the Saccharomyces cerevisiae protein, Msn5p/Kap142p. Like other known …

The nuclear pore complex (NPC) is a 125 000 kDa 1 machine that transports both proteins
and RNAs bidirectionally across the nuclear envelope without leakage, and incorporates …

A hallmark of eukaryotic cells is their separation into compartments. These compartments
are surrounded by membranes that are impermeable to macromolecules. As most proteins …

RNA export is the process by which RNAs are transported to the cytoplasm after synthesis,
processing, and RNP assembly within the nucleus. The primary focus of this review is mRNA …

We recently developed an assay in which nuclear export of the shuttling transcription factor
NFAT (nuclear factor of activated T cells) can be reconstituted in permeabilized cells with the …