Sensitive and Rapid Diagnosis of Respiratory Virus Coinfection Using a Microfluidic Chip‐Powered CRISPR/Cas12a System

J Liu, H Wang, L Zhang, Y Lu, X Wang, M Shen, N Li… - Small, 2022 - Wiley Online Library
The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 is
profoundly influencing the global healthcare system and people's daily lives. The high …

RT-RPA-assisted CRISPR/Cas12a for rapid and multiplex detection of respiratory infectious viruses based on centrifugal microfluidics

R Peng, Z Lu, M Liu, F Hu - Sensors and Actuators B: Chemical, 2024 - Elsevier
Clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid
detection technology combined with recombinase polymerase amplification (RPA) allows for …

CRISPR/Cas12a-Assisted isothermal amplification for rapid and specific diagnosis of respiratory virus on an microfluidic platform

J Shen, Z Chen, R Xie, J Li, C Liu, Y He, X Ma… - Biosensors and …, 2023 - Elsevier
Respiratory viruses have long been a major cause of a global pandemic, emphasizing the
urgent need for high-sensitivity diagnostic tools. Typical PCR technology can only determine …

Rapid detection of SARS-CoV-2 with CRISPR-Cas12a

D Xiong, W Dai, J Gong, G Li, N Liu, W Wu, J Pan… - PLoS …, 2020 - journals.plos.org
The recent outbreak of betacoronavirus Severe Acute Respiratory Syndrome Coronavirus 2
(SARS-CoV-2), which is responsible for the Coronavirus Disease 2019 (COVID-19) global …

Detection of infectious viruses using CRISPR-Cas12-based assay

CS Talwar, KH Park, WC Ahn, YS Kim, OS Kwon… - Biosensors, 2021 - mdpi.com
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which
causes coronavirus disease-19 (COVID-19), has severely influenced public health and …

Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants

NL Welch, M Zhu, C Hua, J Weller, ME Mirhashemi… - Nature medicine, 2022 - nature.com
Abstract The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear
need for high-throughput, multiplexed and sensitive assays for detecting severe acute …

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

P Fozouni, S Son, MD de León Derby, GJ Knott… - Cell, 2021 - cell.com
The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an
ongoing global pandemic due in part to the challenge of identifying symptomatic …

Instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using self-contained microfluidic system

Z Li, X Ding, K Yin, L Avery, E Ballesteros… - Biosensors and …, 2022 - Elsevier
Rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) is critical for early diagnostics and timely medical treatment of coronavirus disease …

Highly sensitive and rapid detection of SARS‐CoV‐2 via a portable CRISPR‐Cas13a‐based lateral flow assay

H Liu, S Chang, S Chen, Y Du, H Wang… - Journal of Medical …, 2022 - Wiley Online Library
To rapidly identify individuals infected with severe acute respiratory syndrome coronavirus‐2
(SARS‐CoV‐2) and control the spread of coronavirus disease (COVID‐19), there is an …

A multiplexed Cas13-based assay with point-of-care attributes for simultaneous COVID-19 diagnosis and variant surveillance

M Patchsung, A Homchan, K Aphicho… - The CRISPR …, 2023 - liebertpub.com
Point-of-care (POC) nucleic acid detection technologies are poised to aid gold-standard
technologies in controlling the COVID-19 pandemic, yet shortcomings in the capability to …