This article presents an overview of time-resolved (lifetime) fluorescence techniques used in
biomedical diagnostics. In particular, we review the development of time-resolved …

Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is
now finding an important place in quantitative imaging of protein–protein interactions and …

This article describes a wide-field time-domain fluorescence lifetime imaging FLIM
microscope with optical sectioning. The FLIM system utilizes a wide-field time-gated optical …

While laser scanning fluorescence lifetime imaging (FLIM) is a powerful approach for cell
biology, its small field of view (typically less than 1 mm) makes it impractical for the imaging …

Förster (or fluorescence) resonance energy transfer (FRET) and fluorescence lifetime
imaging (FLIM) have moved center stage and are increasingly forming part of multifaceted …

The experimental configuration and the computational algorithms for performing multiple
frequency fluorescence lifetime imaging microscopy (mfFLIM) are described. The mfFLIM …

Fluorescence lifetime imaging microscopy (FLIM) and fluorescence anisotropy imaging
microscopy (FAIM) are versatile tools for the investigation of the molecular environment of …

A fast time-domain based fluorescence lifetime imaging (FLIM) microscope is presented that
can operate at frame rates of hundreds of frames per second. A beam splitter in the detection …

Fluorescence lifetime imaging (FLIM) is widely applied to obtain quantitative information
from fluorescence signals, particularly using Förster Resonant Energy Transfer (FRET) …