A pragmatic eLCR for an ultrasensitive detection of methicillin-resistant Staphylococcus aureus in joint synovial fluid: superior to qPCR

J Chen, H Wei, X Fang, Y Cai, Z Zhang, Y Wang, J Lin… - Analyst, 2021 - pubs.rsc.org
For periprosthetic joint infection (PJI) patients, an early and rapid detection of methicillin-
resistant Staphylococcus aureus (MRSA) in joint synovial fluid is of great significance for …

A new multiplex real-time polymerase chain reaction assay for the diagnosis of periprosthetic joint infection

M Kawamura, N Kobayashi, Y Inaba… - Modern …, 2017 - academic.oup.com
Objectives: A new multiplex real-time polymerase chain reaction (PCR) assay was
developed to detect methicillin-resistant Staphylococcus (MRS) and to distinguish between …

Is Xpert MRSA/SA SSTI real-time PCR a reliable tool for fast detection of methicillin-resistant coagulase-negative staphylococci in periprosthetic joint infections?

J Lourtet-Hascoëtt, A Bicart-See, MP Félicé… - … and infectious disease, 2015 - Elsevier
Periprosthetic joint infections (PJIs) are frequently caused by methicillin-resistant coagulase-
negative staphylococci (CoNS). Cultures remain the gold standard but often require a few …

Rapid and sensitive detection of methicillin-resistant Staphylococcus periprosthetic infections using real-time polymerase chain reaction

N Kobayashi, Y Inaba, H Choe, N Iwamoto… - … and infectious disease, 2009 - Elsevier
The aim of this study was to validate the accuracy, sensitivity, and specificity of a methicillin-
resistant Staphylococcus (MRS) real-time polymerase chain reaction (PCR) assay in clinical …

Bacterial detection and identification from human synovial fluids on an integrated microfluidic system

TH Liu, SS Cheng, HL You, MS Lee, GB Lee - Analyst, 2019 - pubs.rsc.org
Periprosthetic joint infections (PJIs) are among the most severe complications emerging
from prosthetic joint replacement surgeries. In order to possess a rapid means of diagnosing …

An automated real‐time PCR assay for synovial fluid improves the preoperative etiological diagnosis of periprosthetic joint infection and septic arthritis

F Yang, H Choe, N Kobayashi, T Tezuka… - Journal of …, 2021 - Wiley Online Library
Synovial fluid is important for the preoperative etiological diagnosis of suspected
periprosthetic joint infection (PJI) or septic arthritis (SA). GENECUBE, an automated real …

An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus

L Xu, W Liang, Y Wen, L Wang, X Yang, S Ren… - Biosensors and …, 2018 - Elsevier
Electrochemical DNA biosensor has unique advantages for on-site pathogenic
microorganism detection, yet the detection of long DNA towards genome DNA (gDNA) …

[PDF][PDF] Intraoperative diagnosis of Staphylococcus aureus and coagulase-negative Staphylococcus using Xpert MRSA/SA SSTI assay in prosthetic joint infection

A Sambri, G Pignatti, M Romagnoli, D Donati… - New …, 2017 - newmicrobiologica.org
The Xpert® MRSA/SA SSTI assay (Cepheid, Sunnyvale, CA, USA) performed on the
GeneXpert® instrument is a new generation test based on multiplex Real Time-PCR …

Evaluation of rapid mecA gene detection versus standard culture in staphylococcal chronic prosthetic joint infections

M Titécat, C Loïez, E Senneville, F Wallet… - … and infectious disease, 2012 - Elsevier
In case of periprosthetic joint infections, the antibiotic treatment administered intraoperatively
entails consequences on bacterial ecology with potential secondary effects. This study …

[HTML][HTML] Enrichment of bacteria samples by centrifugation improves the diagnosis of orthopaedics-related infections via real-time PCR amplification of the bacterial …

A Tsuru, T Setoguchi, N Kawabata, M Hirotsu… - BMC research …, 2015 - Springer
Background To effectively treat orthopaedic infections by methicillin-resistant strains, an
early diagnosis is necessary. Bacterial cultures and real-time polymerase chain reaction …