Quantifying bound and active antibodies conjugated to gold nanoparticles: A comprehensive and robust approach to evaluate immobilization chemistry

K Tripathi, JD Driskell - ACS omega, 2018 - ACS Publications
Gold nanoparticles (AuNPs) functionalized with antibodies have the potential to improve
biosensing technology because of the unique optical properties of AuNPs and the specificity …

High-affinity points of interaction on antibody allow synthesis of stable and highly functional antibody–gold nanoparticle conjugates

S Okyem, O Awotunde, T Ogunlusi… - Bioconjugate …, 2021 - ACS Publications
Many emerging nanobiotechnologies rely on the proper function of proteins immobilized on
gold nanoparticles. Often, the surface chemistry of the AuNP is engineered to control the …

pH impacts the orientation of antibody adsorbed onto gold nanoparticles

G Ruiz, K Tripathi, S Okyem, JD Driskell - Bioconjugate chemistry, 2019 - ACS Publications
Novel detection strategies that exploit the unique properties of gold nanoparticles (AuNPs)
hold great promise for the advancement of diagnostic testing. Fundamental to many of these …

Highly robust and optimized conjugation of antibodies to nanoparticles using quantitatively validated protocols

S Jeong, JY Park, MG Cha, H Chang, Y Kim, HM Kim… - Nanoscale, 2017 - pubs.rsc.org
Antibody-conjugated nanoparticles (NPs) have attracted great attention in diagnostic and
therapeutic applications due to their high sensitivity and specificity for biotargets, as well as …

Antibody-gold nanoparticle bioconjugates for biosensors: synthesis, characterization and selected applications

L Zhang, Y Mazouzi, M Salmain, B Liedberg… - Biosensors and …, 2020 - Elsevier
Abstract Antibody-Gold nanoparticle (Ab-AuNP) bioconjugates are widely used in the field of
biosensing. This prompted researchers to set up various strategies to conjugate antibodies …

Optimization and structural stability of gold nanoparticle–antibody bioconjugates

RT Busch, F Karim, J Weis, Y Sun, C Zhao… - ACS …, 2019 - ACS Publications
Gold nanoparticles (AuNPs) bound with biomolecules have emerged as suitable biosensors
exploiting unique surface chemistries and optical properties. Many efforts have focused on …

Functionalization of gold nanoparticles with nanobodies through physical adsorption

J Goossens, H Sein, S Lu, M Radwanska… - Analytical …, 2017 - pubs.rsc.org
Nanobodies (Nbs) are single-domain antibodies with highly desirable properties employed
as versatile tools in structural biology, biochemistry and biomedical sciences. Nbs also have …

Direct quantification of surface coverage of antibody in IgG-Gold nanoparticles conjugates

L Zhang, D Hu, M Salmain, B Liedberg, S Boujday - Talanta, 2019 - Elsevier
It is of paramount importance to be able to accurately quantify surface coverage of
antibodies on gold nanoparticles (AuNP) so as to optimise the sensitivity of AuNP-based …

Design, preparation, and evaluation of a fixed-orientation antibody/gold-nanoparticle conjugate as an immunosensing label

C Parolo, A de la Escosura-Muñiz, E Polo… - … applied materials & …, 2013 - ACS Publications
Herein, we describe the development of a new, highly efficient label for immunosensing
comprising an antibody/PEGylated gold-nanoparticle (AuNP) conjugate in which the …

Antibodies irreversibly adsorb to gold nanoparticles and resist displacement by common blood proteins

G Ruiz, N Ryan, K Rutschke, O Awotunde, JD Driskell - Langmuir, 2019 - ACS Publications
Gold nanoparticles (AuNPs) functionalized with proteins to impart desirable surface
properties have been developed for many nanobiotechnology applications. A strong …