Quantifying bound and active antibodies conjugated to gold nanoparticles: A comprehensive and robust approach to evaluate immobilization chemistry
K Tripathi, JD Driskell - ACS omega, 2018 - ACS Publications
Gold nanoparticles (AuNPs) functionalized with antibodies have the potential to improve
biosensing technology because of the unique optical properties of AuNPs and the specificity …
biosensing technology because of the unique optical properties of AuNPs and the specificity …
High-affinity points of interaction on antibody allow synthesis of stable and highly functional antibody–gold nanoparticle conjugates
S Okyem, O Awotunde, T Ogunlusi… - Bioconjugate …, 2021 - ACS Publications
Many emerging nanobiotechnologies rely on the proper function of proteins immobilized on
gold nanoparticles. Often, the surface chemistry of the AuNP is engineered to control the …
gold nanoparticles. Often, the surface chemistry of the AuNP is engineered to control the …
pH impacts the orientation of antibody adsorbed onto gold nanoparticles
Novel detection strategies that exploit the unique properties of gold nanoparticles (AuNPs)
hold great promise for the advancement of diagnostic testing. Fundamental to many of these …
hold great promise for the advancement of diagnostic testing. Fundamental to many of these …
Highly robust and optimized conjugation of antibodies to nanoparticles using quantitatively validated protocols
Antibody-conjugated nanoparticles (NPs) have attracted great attention in diagnostic and
therapeutic applications due to their high sensitivity and specificity for biotargets, as well as …
therapeutic applications due to their high sensitivity and specificity for biotargets, as well as …
Antibody-gold nanoparticle bioconjugates for biosensors: synthesis, characterization and selected applications
L Zhang, Y Mazouzi, M Salmain, B Liedberg… - Biosensors and …, 2020 - Elsevier
Abstract Antibody-Gold nanoparticle (Ab-AuNP) bioconjugates are widely used in the field of
biosensing. This prompted researchers to set up various strategies to conjugate antibodies …
biosensing. This prompted researchers to set up various strategies to conjugate antibodies …
Optimization and structural stability of gold nanoparticle–antibody bioconjugates
Gold nanoparticles (AuNPs) bound with biomolecules have emerged as suitable biosensors
exploiting unique surface chemistries and optical properties. Many efforts have focused on …
exploiting unique surface chemistries and optical properties. Many efforts have focused on …
Functionalization of gold nanoparticles with nanobodies through physical adsorption
J Goossens, H Sein, S Lu, M Radwanska… - Analytical …, 2017 - pubs.rsc.org
Nanobodies (Nbs) are single-domain antibodies with highly desirable properties employed
as versatile tools in structural biology, biochemistry and biomedical sciences. Nbs also have …
as versatile tools in structural biology, biochemistry and biomedical sciences. Nbs also have …
Direct quantification of surface coverage of antibody in IgG-Gold nanoparticles conjugates
L Zhang, D Hu, M Salmain, B Liedberg, S Boujday - Talanta, 2019 - Elsevier
It is of paramount importance to be able to accurately quantify surface coverage of
antibodies on gold nanoparticles (AuNP) so as to optimise the sensitivity of AuNP-based …
antibodies on gold nanoparticles (AuNP) so as to optimise the sensitivity of AuNP-based …
Design, preparation, and evaluation of a fixed-orientation antibody/gold-nanoparticle conjugate as an immunosensing label
Herein, we describe the development of a new, highly efficient label for immunosensing
comprising an antibody/PEGylated gold-nanoparticle (AuNP) conjugate in which the …
comprising an antibody/PEGylated gold-nanoparticle (AuNP) conjugate in which the …
Antibodies irreversibly adsorb to gold nanoparticles and resist displacement by common blood proteins
G Ruiz, N Ryan, K Rutschke, O Awotunde, JD Driskell - Langmuir, 2019 - ACS Publications
Gold nanoparticles (AuNPs) functionalized with proteins to impart desirable surface
properties have been developed for many nanobiotechnology applications. A strong …
properties have been developed for many nanobiotechnology applications. A strong …
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- immobilization chemistry gold nanoparticles
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- igg gold surface coverage
- direct quantification surface coverage
- antibody conjugation immunochromatographic assay
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