Expression and characterization of the acidic subunit from 11S amaranth seed protein

S Luna‐Suárez, S Medina‐Godoy… - Biotechnology …, 2008 - Wiley Online Library
Amarantin acidic subunit has the potential to be employed as a functional and a
nutraceutical protein. To evaluate both possibilities this protein was produced in …

[HTML][HTML] Expression, purification and thermal stability evaluation of an engineered amaranth protein expressed in Escherichia coli

JI Morales-Camacho, O Paredes-López… - Electronic Journal of …, 2016 - SciELO Chile
Background: The acidic subunit of amarantin (AAC)-the predominant amaranth seed storage
protein-has functional potential and its third variable region (VR) has been modified with …

Overexpression, Purification, and in Vitro Refolding of the 11S Globulin from Amaranth Seed in Escherichia coli

JA Osuna-Castro, Q Rascón-Cruz… - Journal of agricultural …, 2000 - ACS Publications
An amarantin 11S globulin cDNA encoding one of the most important storage proteins of
amaranth seeds, with a high content of essential amino acids, was expressed in Escherichia …

Overexpression of a modified protein from amaranth seed in Escherichia coli and effect of environmental conditions on the protein expression

C Castro-Martínez, S Luna-Suárez… - Journal of …, 2012 - Elsevier
Amaranth seeds are considered as an excellent complementary source of food protein due
to their balanced amino acid composition. Amarantin acidic subunit has the potential as a …

One-step purification and structural characterization of a recombinant His-tag 11S globulin expressed in transgenic tobacco

A Valdez-Ortiz, Q Rascón-Cruz, S Medina-Godoy… - Journal of …, 2005 - Elsevier
Amarantin, an 11S globulin, is one of the most important storage proteins of amaranth
seeds, with relevant nutritional–functional and nutraceutical characteristics. Its cDNA was …

Endoplasmic reticulum‐retention C‐terminal sequence enhances production of an 11S seed globulin from Amaranthus hypochondriacus in Pichia pastoris

S Medina‐Godoy, A Valdez‐Ortiz… - Biotechnology …, 2006 - Wiley Online Library
The methylotrophic yeast Pichia pastoris was used to express an 11S seed globulin from
Amaranthus hypochondriacus. Three different plasmids were tested for expression of …

Accumulation, assembly, and digestibility of amarantin expressed in transgenic tropical maize

Q Rascón-Cruz, S Sinagawa-García… - Theoretical and Applied …, 2004 - Springer
An amaranth (Amaranthus hypochondriacus) 11S globulin cDNA, encoding one of the most
important storage proteins (amarantin) of the seed, with a high content of essential amino …

Overexpression of a modified amaranth protein in Escherichia coli with minimal media and lactose as inducer

J Ismael Morales-Camacho… - Recent Patents on …, 2013 - ingentaconnect.com
In this research it was attempted to overexpress the acidic subunit, from the 11S amaranth
seed globulin termed amarantin, modified with antihypertensive peptides in Escherichia coli …

Modification of the amaranth 11S globulin storage protein to produce an inhibitory peptide of the angiotensin I converting enzyme, and its expression in Escherichia …

S Luna-Suárez, S Medina-Godoy… - Journal of …, 2010 - Elsevier
Amarantin is the predominant seed storage protein from amaranth. It shows a high content of
essential amino acids, making this protein important from a nutritional viewpoint. The protein …

Expression and Characterization of a His‐Tagged 11S Seed Globulin from Amaranthus hypochondriacus in Escherichia coli

S Medina‐Godoy, NC Nielsen… - Biotechnology …, 2004 - Wiley Online Library
DNA encoding a His‐tagged 11S globulin from Amaranthus hypochondriacus (amarantin)
was successfully expressed in Escherichia coli strains BL21 (DE3) and Origami (DE3). The …