A readily releasable pool (RRP) of granules has been proposed to underlie the first phase of
insulin secretion. In the present study we combined electron microscopy, insulin secretion …

Density gradient centrifugation techniques, using iso-osmotic colloidal silica suspensions
(Percoll), were developed for the isolation of insulin secretory granules from a transplantable …

Rat pancreatic islets were maintained in tissue culture (8.3 mM glucose) for 3 days in the
presence of [3H] leucine in order to label insulin stores. Alternatively, islets were labeled for …

Beta granules isolated from rat islets of Langerhans and subjected only to phosphotungstic
acid had, in negatively stained images, a 50-A periodicity. This periodicity was also …

The early development of the pancreatic islet organ is intimately associated with the
development of the whole pancreas. The pancreatic primordia first appear as duodenal …

Pancreatic islets have been studied by the electron microscopy of ultrathin sections in
mature guinea pigs and cats. Alpha cells contain specific granules which are notably dense …

The pancreatic islets of the guinea pig have been studied by light and electron microscopy.
The B granules in glutaraldehyde-fixed tissue often are cup-shaped with an indentation …

The electron microscopic study of rabbit pancreatic islets reveals in adthtiun to alpha anti
beta cells a third cell type containing specific secretory graaules. This third cell is considered …

To investigate compartmental properties of insulin storage and secretion, isolated rat islets
were used for pulse-labeling experiments, after which proinsulin and insulin were purified …

When the technique of immunofluorescence is applied to rat pancreas to detect insulin,
glucagon, somatostatin and pancreatic polypeptide (PP), two populations of islets having …