Isolated rat pancreatic islets were pulse-labeled for 5 min with [3H] leucine then chased for
25 min, during which time endogenously labeled [3H] proinsulin becomes predominantly …

A zymogen granule fraction has been isolated from rat pancreas, and its purity has been
assessed by biochemical and morphological criteria. Specific activities of two marker …

The synthesis, intracellular transport, storage, and discharge of secretory proteins in and
from the pancreatic exocrine cell of the guinea pig were studied by light-and electron …

Publisher Summary This chapter presents the concept of the involvement of the microtubular-
microfilamentous system in the intracellular transport of beta granules and the mechanism …

1. An in vitro system is described in which insulin beta‐granule‐plasma membrane
interaction can be studied. 2. The system shows an absolute requirement for physiological …

Isolated pancreatic zymogen granules have been reported to lyse in common electrolyte
solutions such as NaCl or KCl or at pH values above 5.5. A new method, based on an …

In the first descriptions of pancreatic enzyme secretion about 100 years ago, it was noticed
that zymogen granules became smaller and disappeared from the apical region of acinar …

Electron microscopic criteria have been established for the identification of all the different
types of islet cells. A remarkable difference in the ultrastructure of beta granules has been …

The zymogen granules have been isolated from dog pancreas in relatively pure form. They
contain very small amounts of ribonucleic acid and phospholipid. On a nitrogen basis they …

The role of Ca2+ in initiating exocytosis of granule-bound secretory products was evaluated
with respect to pancreatic islet hormones. Apart from stimulating the transfer of the granules …