Targeted genome editing with engineered nucleases has transformed the ability to introduce
precise sequence modifications at almost any site within the genome. A major obstacle to …

New technologies to target nucleotide diversification in vivo are promising enabling
strategies to perform directed evolution for engineering applications and forward genetics for …

The availability of genome sequences of numerous organisms and the revolution brought
about by genome editing tools (eg, ZFNs, TALENs, and CRISPR/Cas9 or RGENs) has …

Clustered regularly interspaced short palindromic repeats (CRISPR) technology has greatly
accelerated the field of strain engineering. However, insufficient efforts have been made …

To the Editor: RNA editing, the post-transcriptional modification of genomic information,
results in variation that can be observed in the transcriptome. In mammals, only two types of …

In many organisms of biotechnological importance precise genome editing is limited by
inherently low homologous recombination (HR) efficiencies. A number of strategies exist to …

Knockout experiments are critical for the evaluation of gene function. Researchers have
increasingly relied on genome editing technologies for precise mutagenesis at loci of …

CRISPR-Cas9-based genome editing enables the rapid genetic manipulation of any
genomic locus without the need for gene targeting by homologous recombination. Here we …

The insertion of precise genetic modifications by genome editing tools such as CRISPR-
Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared …

Genome editing holds promise for correcting pathogenic mutations. However, it is difficult to
determine off-target effects of editing due to single-nucleotide polymorphism in individuals …